1.1.1.87	R85V	complete loss of activity with isocitrate, significant activity with 3-isopropylmalate, no effect on activity with homoisocitrate	-, 656127	
1.1.1.87	A80del	site-directed mutagenesis, the mutant shows altered substrate specificity preferring isocitrate to homoisocitrate, it is unable to oxidize 3-isopropylmalate, the specificity is similar to the enzyme from Thermus thermophilus	660918	
1.1.1.87	additional information	modulation of the broad substrate specificity of the trifunctional enzyme through site-directed mutagenesis, overview	660918	
1.1.1.87	R87T	site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate	660918	
1.1.1.87	R87V	site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate	660918	
1.1.1.87	V135M	site-directed mutagenesis, tetramer-to-dimer structural transition enhances the activity with isocitrate 1.6fold	669109	
1.1.1.87	additional information	trex mutant allele, T to C exchange at nucleotide 251. Midgestation lethal mutant which displays craniofacial, limb, and organ abnormalities due to insufficient retinoic acid signaling	686950	
1.1.1.87	K206M	site-directed mutagenesis, the active site mutant shows about 2400fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly	696350	
1.1.1.87	Y150F	site-directed mutagenesis, the active site mutant shows about 680fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly	696350	
1.1.1.87	D271N	site-directed mutagenesis, mutation of a metal ion ligand and binding determinant for Mg2+, to N. The mutant enzyme shows a decrease of 520fold in V and V/Km_Mg2+, suggesting that the same step(s) limit the reaction at limiting and saturating MgHIc concentrations	739958