1.1.1.53	I15T	in 20beta-HSD type A, cofactor binding is abolished	287107	
1.1.1.53	Q27K	in 20beta-HSD type B, cofactor binding is reduced	287107	
1.1.1.53	T15I	in 20beta-HSD type B, enzyme activity is restored	287107	
1.1.1.53	I6T/V47M/T69K	mutant enzyme with a maximal improvement in solubility, high catalytic activity as a NADP-dependent 3alpha,20beta-hydroxysteroid dehydrogenase	-, 657157	
1.1.1.53	I6T/V47M/T69K/E142A	pH optimum for reduction of progesterone shifts slightly from pH 6.0 to pH 6.5 compared to for mutant enzyme I6T/V47M/T69K, the oxidation of androsterone shows a dual pH optimum, one at pH 6.0-6.5 and the other at pH 10	-, 657157	
1.1.1.53	I6T/V47M/T69K/S140A	both oxidation of androsterone and reduction of progesterone are completely lost	-, 657157	
1.1.1.53	I6T/V47M/T69K/Y153F	both oxidation of androsterone and reduction of progesterone are completely lost	-, 657157	
1.1.1.53	V54L	the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme	738928