7.5.2.5	E163Q	site-directed mutagenesis, catalytically inactive variant, introduction of plasmid-encoded LptBE163Q-His8 variant into the wild-type strain results in increased outer membrane permeability, dominant-negative phenotype	752031	
7.5.2.5	F90A	site-directed mutagenesis, catalytically inactive variant, the F90A variant cannot form a stable complex with the Lpt-inner membrane components	752031	
7.5.2.5	F90Y	site-directed mutagenesis, the LptB and LptB-His8 F90Y variants are functional in vivo	752031	
7.5.2.5	H195A	site-directed mutagenesis, catalytically inactive variant, introduction of plasmid-encoded LptB-H195A-His8 variant into the wild-type strain results in increased outer membrane permeability, dominant-negative phenotype	752031	
7.5.2.5	additional information	amorphadiene, the precursor of antimalarial drug artemisinin from Artemisia annua, is secreted from Escherichia coli cells overexpressing the biosynthetic pathway. The overexpression of transporters in the lipopolysaccharide transport system (msbA, lptD, lptCABFG) improves amorphadiene (AD) production. AD production in both early stage (8 h) and final stage (24 h) is increased by more than twofold in the strains that overexpress lptCABFG or msbA. But co-overexpression of LptCABFG and LptD or LptD and TolC does not enhance AD-specific production synergistically, despite the fact that the AD titer is increased mainly due to the increased cell density, overview	750126	
7.5.2.5	additional information	construction of a several HB-1DELTAlptB lptB deletion mutants, phenotypes, overview. The expression of PagL in the lptH, lptC, lptF, and lptG mutants does not affect LPS mobility, demonstrating that the residual LPS present in these strains does not reach the cell surface. PagL is an outer membrane (OM) enzyme that can remove an acyl chain from LPS when LPS is present at its proper location, i.e. the outer leaflet of the OM	-, 751032	
7.5.2.5	additional information	construction of chromosomal deletion of lptB and replacement with a kanamycin-resistance cassette (DELTAlptB:: kan allele), where the region from the second codon to the stop codon of lptB is deleted	752031