4.2.1.108	F107A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	F107W	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	F107Y	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H117A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	-, 749081	
4.2.1.108	H51A	site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme	749081	
4.2.1.108	H55A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H93A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H93N	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	L87A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	additional information	structure-guided site-directed mutagenesis is used targeting amino acid residues that are evolutionarily highly conserved among the extended EctC protein family, including those forming the presumptive iron-binding site	-, 749081