4.2.1.108	C105A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	-, 749081	
4.2.1.108	C105S	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	-, 749081	
4.2.1.108	D91A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	D91E	site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme	749081	
4.2.1.108	E115A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	E115D	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	E57A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	-, 749081	
4.2.1.108	E57D	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	F107A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	F107W	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	F107Y	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H117A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	-, 749081	
4.2.1.108	H51A	site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme	749081	
4.2.1.108	H55A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H93A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	H93N	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	L87A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	additional information	construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique	-, 713911	
4.2.1.108	additional information	expression in transgenic Nicotiana tabacum BY2 cells increases the osmotolerance and growth rate of the cells under hyperosmotic conditions	-, 663114	
4.2.1.108	additional information	structure-guided site-directed mutagenesis is used targeting amino acid residues that are evolutionarily highly conserved among the extended EctC protein family, including those forming the presumptive iron-binding site	-, 749081	
4.2.1.108	S23A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	T40A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	T41A	site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme	749081	
4.2.1.108	W21A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	Y52A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	Y85A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	Y85F	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081	
4.2.1.108	Y85W	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	749081