4.2.1.108 F107Y site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 H117A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme -, 749081 4.2.1.108 H51A site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme 749081 4.2.1.108 H55A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 H93A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 H93N site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 L87A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 additional information construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique -, 713911 4.2.1.108 additional information expression in transgenic Nicotiana tabacum BY2 cells increases the osmotolerance and growth rate of the cells under hyperosmotic conditions -, 663114 4.2.1.108 additional information structure-guided site-directed mutagenesis is used targeting amino acid residues that are evolutionarily highly conserved among the extended EctC protein family, including those forming the presumptive iron-binding site -, 749081