4.1.1.102 D112N 121% of wild-type activity -, 733201 4.1.1.102 E134A mutation decreases the enzyme activity by more than 60% -, 735040 4.1.1.102 E285A loss of catalytic activity 733178 4.1.1.102 F95A 120% of wild-type activity -, 733201 4.1.1.102 F95A/D112N 395% of wild-type activity 733201 4.1.1.102 F95A/S157I 218% of wild-type activity 733201 4.1.1.102 F95A/V151I 333% of wild-type activity 733201 4.1.1.102 F95E 26% of wild-type activity 733201 4.1.1.102 F95I 148% of wild-type activity 733201 4.1.1.102 F95I/D112N 418% of wild-type activity 733201 4.1.1.102 F95I/S157I 299% of wild-type activity 733201 4.1.1.102 F95I/V151I 323% of wild-type activity 733201 4.1.1.102 F95K 18% of wild-type activity 733201 4.1.1.102 F95L 188% of wild-type activity -, 733201 4.1.1.102 F95L/D112N 485% of wild-type activity 733201 4.1.1.102 F95L/D112N/S157I 430% of wild-type activity 733201 4.1.1.102 F95L/D112N/V151I 1354% of wild-type activity -, 733201 4.1.1.102 F95L/D112N/V151I approximately 34fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid, possibly due to formation of a compact active site compared with that of the wild-type -, 733201 4.1.1.102 F95L/S157I 528% of wild-type activity 733201 4.1.1.102 F95L/V151I 746% of wild-type activity 733201 4.1.1.102 F95L/V151I/S157I 484% of wild-type activity 733201 4.1.1.102 F95Q 48% of wild-type activity 733201 4.1.1.102 F95R 19% of wild-type activity 733201 4.1.1.102 M57A almost complete loss of activity -, 733069 4.1.1.102 M57L mutation does not increase the ratio of decarboxylation activity toward ferulate to 4-coumarate -, 733069 4.1.1.102 M57T almost complete loss of activity -, 733069 4.1.1.102 S157I 171% of wild-type activity 733201 4.1.1.102 V151I 174% of wild-type activity 733201 4.1.1.102 W25A mutation decreases the enzyme activity by more than 95% -, 735040 4.1.1.102 Y21A mutation abolishes the enzyme activity completely -, 735040 4.1.1.102 Y27A mutation abolishes the enzyme activity completely -, 735040