3.7.1.4	A126S	site-directed mutagenesis	735304	
3.7.1.4	A213S	the mutant completely loses catalytic activity towards phloretin	750528	
3.7.1.4	E164A	the mutation completely abolishes the hydrolytic activity towards phloretin	-, 750528	
3.7.1.4	E273A	the mutation completely abolishes the hydrolytic activity towards phloretin	-, 750528	
3.7.1.4	F218Y	site-directed mutagenesis	735304	
3.7.1.4	H118A	the mutant completely loses catalytic activity towards phloretin	-, 750528	
3.7.1.4	H133A	the kcat value for phloretin is decreased at least by 1 order of magnitude, whereas the corresponding Km value is barely affected compared to the wild type enzyme	750528	
3.7.1.4	H160A	the mutant completely loses catalytic activity towards phloretin	-, 750528	
3.7.1.4	H160F	the mutant completely loses catalytic activity towards phloretin	-, 750528	
3.7.1.4	H217A	the mutant has a Km value increased by 3fold and a kcat value decreased by 6fold compared to the wild type enzyme	750528	
3.7.1.4	H269A	the kcat value for phloretin is decreased at least by 1 order of magnitude, whereas the corresponding Km value is barely affected compared to the wild type enzyme	750528	
3.7.1.4	I162A	the mutant completely loses catalytic activity towards phloretin	750528	
3.7.1.4	additional information	deletions of the LPH 5' flanking sequence and fusion 5' to the human growth hormone reporter gene	675587	
3.7.1.4	additional information	Gata4 mutant, LPH mRNA abundance is significantly attenuated	675975	
3.7.1.4	additional information	hypolactasia seems to be strongly corretated with genotype C/C of the genetic variant C->T-13910 upstream of the lactase-phlorizin hydrolase gene	673123	
3.7.1.4	additional information	mutation of His123, His251, Glu154 and Glu255 (conserved zinc binding residues) results in variants that were either poorly expressed, or of much reduced activity. Mutation of Tyr115 and His203, thought to bind the phenol groups in the 1-and 3-positions of the phloroglucinol ring respectively, results in variants of 15-fold reduced activity and an inactive variant	735304	
3.7.1.4	additional information	the genetic variant C/T-13910 upstream of the lactase-phlorizin hydrolase gene is strongly correlated with the lactase persistence/nonperistence trait	687838	
3.7.1.4	additional information	the mutations C/T-13910 and G/A-22018 of the lactase-phlorizin hydrolase gene are correlated with lactose absorption	685892	
3.7.1.4	N1340Q	the variant lacks the closest potential N-glycosylation site to Asp1338	749964	
3.7.1.4	N1814Q	the variant lacks the N-glycosylation site in the domain IV	749964	
3.7.1.4	N821Q	the variant lacks the N-glycosylation site in the domain II	749964	
3.7.1.4	Q266F	the mutant completely loses catalytic activity towards phloretin	750528	
3.7.1.4	Y125A	the mutant completely loses catalytic activity towards phloretin	750528	
3.7.1.4	Y232A	the Km value for phloretin increases by 3fold and the kcat value decreases by 5fold compared to the wild type enzyme	750528