3.5.99.7	E295D	site-directed mutagesis, mutant kinetics compared to the wild-type, overview	-, 718894	
3.5.99.7	E295S	by site-directed mutagenesis, the Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template	-, 694780	
3.5.99.7	E295S/L322T	the double mutant is constructed using the E295S mutant as the template	-, 694780	
3.5.99.7	E295S/L322T	the mutations lead to a loss of enzyme activity against 1-aminocyclopropane-1-carboxylate	-, 735145	
3.5.99.7	G44D	completely inactive, CD spectrum is identical to wild-type	-, 667784	
3.5.99.7	K51T	crystal structure in complex with substrate	648271	
3.5.99.7	additional information	ACC deaminase enhances Agrobacterium tumefaciens-mediated gene transfer into plant cells, e.g. of Cucumis melo var. cantaloupensis or Arabidopsis thaliana, by converting the direct precursor for ethylene, which inhibits gene transfer, into 2-oxobutanoate, overview	-, 684575	
3.5.99.7	additional information	acdS gene deletion mutagenesis, Sinorhizobium sp. BL3-enhancing ACC deaminase activity (BL3+) and defective mutant (BL3-) strains are constructed in order to investigate the effects of this enzyme on symbiosis and nodule senescence. Modulation competitiveness is weaker in BL3- than in the wild-type, but is stronger in BL3+. The inoculation of BL3- into mung bean results in less plant growth, a lower nodule dry weight, and smaller nodule number than those in the wild-type, whereas the inoculation of BL3+ has no marked effects	757612	
3.5.99.7	additional information	compared with the wild-type A1501, the acdS mutant A1815 is more sensitive to the environmental stresses of salt and heavy metal shock. The survival rate of A1815 is lowered by 1-2 orders of magnitude compared with the wild-type when exposed to 1.0 M NaCl treatment for 3 h and by 2-3 orders of magnitude when exposed to 13.2 mM NiCl2 for 3 h	-, 757414	
3.5.99.7	additional information	construction and complementation of acdS and lrpL mutant strains with wild-type strain D3 ACC deaminase, phenotypes, overview	-, 719206	
3.5.99.7	additional information	construction of a disruption mutant deficient of enzyme activity by insertion of a tetracycline resistance gene into the the coding region of the chromosomal copy of the ACC deaminase gene	-, 679006	
3.5.99.7	additional information	construction of a mutant Pseudomonas migulae strain 8R6 lacking the ACC deaminase activity. The bacterial wild-type strain 8R and its mutant lacking the ACC deaminase activity do not promote the growth of either the infected or uninfected plants	-, 757954	
3.5.99.7	additional information	gene acdS expressing mutant strains are generated from ACCD negative psychrotolerant bacterial strains Flavobacterium sp. OR306 and Pseudomonas frederiksbergensis OS211, isolated from agricultural soil during late winter. After transformation with plasmid pRKACC-acdS, both the strains are able to exhibit ACCD activity in vitro. On exposing tomato plants inoculated with both acdS expressing and wild-type bacteria to one week of chilling treatment at 12/10°C, stress ethylene, ACC accumulation and ACO activity, which are markers of ethylene stress, are significantly reduced in plants inoculated with the acdS gene transformed mutants. In case of plants inoculated with strain OS211-acdS, ethylene emission, ACC accumulation and ACO activity are significantly reduced by 52%, 75.9%, and 23.2%, respectively, compared to uninoculated control plants. Expression analysis of cold-induced LeCBF1 and LeCBF3 genes shows that these genes in the plants are significantly induced by the acdS transformed mutant bacteria in addition to observed reduced expression of ethylene-responsive transcription factor 13 (ETF-13) and ACO genes	-, 758070	
3.5.99.7	additional information	gene-disruption mutant, decreased nodulation efficiency with Pisum sativum	648267	
3.5.99.7	additional information	generation of a Streptomyces sp. GMKU 336 ACCD-deficient gene disruption mutant, the mutant strain is catalytically inactive	758431	
3.5.99.7	additional information	random mutagenesis generating a library of mutants for the the elucidation of structure-function relationships	34580	
3.5.99.7	Y268F	site-directed mutagesis, mutant kinetics compared to the wild-type, overview	-, 718894	
3.5.99.7	Y294F	site-directed mutagesis, mutant kinetics compared to the wild-type, overview	-, 718894	
3.5.99.7	Y295F	crystal structure in complex with substrate	648271