3.5.1.52 A208C inactive 712451 3.5.1.52 A208C/Y235H inactive 712451 3.5.1.52 C165T inactive 720808 3.5.1.52 C165T/N166V/R167C inactive 720808 3.5.1.52 C191A catalytically inactive mutant enzyme 689305 3.5.1.52 C191A inactive 720808 3.5.1.52 C191A mutant enzyme does not bind to the inhibitor Man9GlcNAc2-iodoacetoamide 687514 3.5.1.52 C191A no enzymic activity 652230 3.5.1.52 C191A site-directed mutagenesis, inactive mutant 668628 3.5.1.52 C191A site-directed mutagenesis, inactive mutant, inhibitor Z-VAD-fmk does not bind to the mutant enzyme 668234 3.5.1.52 C221T mutant with more than 50% wild type activity 712451 3.5.1.52 C251A the mutant exhibits a defect in degrading RTADELTA-transmembrane-Leu2 protein. Expression of the catalytic mutant results in significant stabilization of RTADELTA protein by binding to N-glycans on the substrate 718978 3.5.1.52 C306A site-directed mutagenesis, inactive mutant, inhibitor Z-VAD-fmk does not bind to the mutant enzyme 668234 3.5.1.52 D179E/P180A mutant with more than 50% wild type activity 712451 3.5.1.52 D208R/V209A mutant with 10-50% wild type activity 712451 3.5.1.52 D217A mutation in the peptide binding site 698128 3.5.1.52 D235A no enzymic activity 652230 3.5.1.52 D235A site-directed mutagenesis, inactive mutant 668628 3.5.1.52 E185R mutant with 10-50% wild type activity 712451 3.5.1.52 E185R/T186V mutant with 10-50% wild type activity 712451 3.5.1.52 E193D inactive 712451 3.5.1.52 E193D/W194F inactive 712451 3.5.1.52 E222A no enzymic activity 652230 3.5.1.52 E238A mutation in the chitobiose binding site 698128 3.5.1.52 F224Y mutant with more than 50% wild type activity 712451 3.5.1.52 G206D/L207I mutant with 10-50% wild type activity 712451 3.5.1.52 G79/F80A site-directed mutagenesis, the double mutation completely abolishes the interaction of PNGase with p97 670704 3.5.1.52 H218A mutation in the chitobiose binding site 698128 3.5.1.52 H218A no enzymic activity 652230 3.5.1.52 H218A site-directed mutagenesis, inactive mutant 668628 3.5.1.52 H218F mutation in the chitobiose binding site 698128 3.5.1.52 H218Y site-directed mutagenesis, the mutant is inactive in protein glycosylation, but interacts with protein Rad23 669579 3.5.1.52 I181W mutant with more than 50% wild type activity 712451 3.5.1.52 I181W/K182Q inactive 712451 3.5.1.52 K182Q mutant with more than 50% wild type activity 712451 3.5.1.52 K253A mutation in the chitobiose binding site 698128 3.5.1.52 L198V mutant with more than 50% wild type activity 712451 3.5.1.52 L200M mutant with more than 50% wild type activity 712451 3.5.1.52 L200M/I201L mutant with more than 50% wild type activity 712451 3.5.1.52 L66M/L75M/L87M site-directed mutagenesis, the PUB domain mutant shows slightly reduced p97 binding 669486 3.5.1.52 additional information complete deletion of aPNGase activity -, 753034 3.5.1.52 additional information construction of PNGase U-373MG siRNA cell lines, the glycosylated type I membrane protein class I MHC HC, dislocated by US2 protein, becomes cytosolic when peptide: N-glycanase is compromised, overview 668402 3.5.1.52 additional information construction of truncated enzyme variants comprising amino acid residues 1-171, 1-471, 471-651, 171-471, 1-111, 112-450, 451-651, and 1-450 670678 3.5.1.52 additional information construction of truncated enzyme variants comprising amino acid residues 1-80, 35-81, 1-181, 1-181DELTAPUB, 1-130, 1-111, 112-450, and 451-651 670704 3.5.1.52 additional information deletion of the N-terminal H1 helix (Png1p-DH1) enhances the deglycosylation activity of peptide:N-glycanase towards denatured glycoproteins 713354 3.5.1.52 additional information enzyme deletion strain, formation of free oligosaccharides is reduced by 70-80% 649758 3.5.1.52 additional information generation of a At3g14920 gene knockout mutant, complete deletion of aPNGase activity in F1 self plants, screened for At3g14920/At5g05480 double-knockout, from Arabidopsis Columbia T-DNA insertion mutant lines SALK_011366 (At3g14920) and SALK_018420 (At5g05480) -, 753034 3.5.1.52 additional information generation of a At5g05480 gene knockout mutant, complete deletion of aPNGase activity in F1 self plants, screened for At3g14920/At5g05480 double-knockout, from Arabidopsis Columbia T-DNA insertion mutant lines SALK_011366 (At3g14920) and SALK_018420 (At5g05480) -, 753034 3.5.1.52 additional information generation of a Ddpngase gene disruption construct, a png-/Ax2 knockout strain, knockout mutants show defect in aggregation -, 733566 3.5.1.52 additional information site-directed mutagenesis of not conserved amino acids 652230 3.5.1.52 N166V/R167C the mutant shows 79% of wild type activity 720808 3.5.1.52 N178A mutation in the peptide binding site 698128 3.5.1.52 N178T mutant with 10-50% wild type activity 712451 3.5.1.52 N214C/R215Q mutant with 10-50% wild type activity 712451 3.5.1.52 N266F/F227H mutant with more than 50% wild type activity 712451 3.5.1.52 N41P site-directed mutagenesis, the mutation completely abolishes the interaction of PNGase with p97 670704 3.5.1.52 N58A site-directed mutagenesis, the mutation does not affect the interaction of PNGase with p97 670704 3.5.1.52 Q239A mutation near the nonreducing end of the chitobiose, possible mannose binding site 698128 3.5.1.52 Q243A mutation near the nonreducing end of the chitobiose, possible mannose binding site 698128 3.5.1.52 R176A mutation in the peptide binding site 698128 3.5.1.52 R187K/K188R mutant with more than 50% wild type activity 712451 3.5.1.52 R210A no enzymic activity 652230 3.5.1.52 V219L mutant with 1-10% wild type activity 712451 3.5.1.52 W123A mutation in the peptide binding site 698128 3.5.1.52 W194F/C195A mutant with 10-50% wild type activity 712451 3.5.1.52 W220F 81% of activity compared to wild type 652230 3.5.1.52 W231F 83% of activity compared to wild type 652230 3.5.1.52 W251A mutation in the chitobiose binding site 698128 3.5.1.52 Y211W mutant with more than 50% wild type activity 712451 3.5.1.52 Y223I mutant with 10-50% wild type activity 712451 3.5.1.52 Y235H inactive 712451