3.4.24.85	D148A	mutant exhibits markedly compromised protease activity	682860	
3.4.24.85	D402N	abolished activity	644083	
3.4.24.85	D467N	mutation of aspartate abolish activity suggesting that is the third residue that coordinates the zinc active site of S2P	644080	
3.4.24.85	E23Q	abolished activity	644083	
3.4.24.85	E55A	mutant exhibits markedly compromised protease activity	682860	
3.4.24.85	H22F	abolished activity	644083	
3.4.24.85	H22F	active site mutant	720919	
3.4.24.85	H26F	abolished activity	644083	
3.4.24.85	H54A	mutant exhibits markedly compromised protease activity	682860	
3.4.24.85	H54A/D148A	mutant exhibits markedly compromised protease activity	682860	
3.4.24.85	H58A	mutant exhibits markedly compromised protease activity	682860	
3.4.24.85	additional information	M19 mutant cell line of CHO-K1 cells with no s2p activity	644079	
3.4.24.85	additional information	mutants in any of the histidines for phenylalanine or glutamic acid for alanine of the consensus metal-binding site abolished activity	644079	
3.4.24.85	N337Q	mutation at glycosylation site N337 does not abolish activity	644080	
3.4.24.85	N508Q	mutation does not abolish activity	644080