3.4.22.B75 C745K mutation does not alter deconjugation rates in comparison with wild-type SENP7 715494 3.4.22.B75 C979S site-directed mutagenesis 732566 3.4.22.B75 C992A site-directed mutagenesis, inactive catalytic mutant 731715 3.4.22.B75 F709W end point deconjugation reactions using di-SUMO2/3 or poly-SUMO2/3 reveales rates equivalent to or slightly greater than wild-type SENP7. Mutation results in a 2fold higher activity when di-SUMO2 deconjugation rates are measured 715494 3.4.22.B75 additional information Depletion of SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 (miSENP7) and a GFP mRNA to enable identification of the transfected cells 732566 3.4.22.B75 additional information enzyme knockout by siRNA in HeLa cells, complementation of knockdown with siRNA-resistant Flag-SENP7 731715 3.4.22.B75 additional information knockdown strategy to deplete SENP7 from NIH-3T3 cells by transfecting a plasmid encoding both a microRNA to downregulate SENP7 and GFP mRNA to enable the identification of microRNA-expressing cells 731614 3.4.22.B75 additional information lentivirus mediated SENP7 enzyme knockdown 731599 3.4.22.B75 V713E mutation elicits 65-fold reduction in deconjugation rate compared to wild-type activity 715494 3.4.22.B75 V713E/C745K C745K substitution partially rescues defects observed for SENP7-V713E when present as a double point substitution (SENP7-V713E/C745K) 715494