3.2.1.25	D199A	low activity	680752	
3.2.1.25	D206N	catalytically active mutant enzyme, similar temperature optimum like wild-type enzyme. The high-catalytic turn-over rate by D206N for beta-glucosidase activity makes it a useful enzyme in cellulose degradation at high temperatures	750443	
3.2.1.25	D206N	in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 11fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 60fold higher than that for 4-nitrophenyl beta-D-mannopyranoside	-, 654641	
3.2.1.25	D206Q	mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides	750443	
3.2.1.25	E206G	complete loss of activity	695664	
3.2.1.25	E207S	no hydrolytic activity	750443	
3.2.1.25	E314G	complete loss of activity	695664	
3.2.1.25	E330A	mutant enzyme displays no activity against mannan or 4-nitrophenyl-beta-mannopyranoside	665524	
3.2.1.25	E399S	no hydrolytic activity	750443	
3.2.1.25	E462A	low activity	680752	
3.2.1.25	E555A	low activity	680752	
3.2.1.25	E555Q	molecular modeling and electron densitiy studies of the Michaelis complex	708104	
3.2.1.25	additional information	mutation associated with caprine beta-mannosidosis: single-base deletion at position 1398 of the coding sequence results in a shift in the reading frame, yielding a deduced peptide of 481 amino acids	136037	
3.2.1.25	N461A	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	N461A	low activity	680752	
3.2.1.25	Q646A	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	Q646A	low activity	680752	
3.2.1.25	Q77R	in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 2fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 1.1fold higher than that for 4-nitrophenyl beta-D-mannopyranoside	-, 654641	
3.2.1.25	Q77R	mutant enzyme shows less than 10% hydrolytic activity than the wild-type toward 4-nitrophenyl glycosides	750443	
3.2.1.25	Q77R/D206N	in contrast to the wild-type enzyme which shows a catalytic efficiency for 4-nitrophenyl beta-D-mannopyranoside that is 5fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 4fold higher than that for 4-nitrophenyl beta-D-glucopyranoside, the mutant enzyme shows highest catalytic efficiency towards 4-nitrophenyl beta-D-glucopyranoside, 8fold higher than that for 4-nitrophenyl beta-D-galactopyranoside and 1.4fold higher than that for 4-nitrophenyl beta-D-mannopyranoside	-, 654641	
3.2.1.25	R641H	natural mutant identified in a patient with beta-mannosidisis. Patient is homozygous for the mutation, which leads to a residual activity of about 7% in the patient's leukocytes, 11% in lymphoblasts and 14% in plasma. Expression in transfected cells also results in 7% residual activity	707885	
3.2.1.25	W198A	low activity	680752	
3.2.1.25	W198G	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	W200A	low activity	680752	
3.2.1.25	W345A	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	W395A	low activity	680752	
3.2.1.25	W645A	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	W645A	low activity	680752	
3.2.1.25	Y537A	crystallization data. Involved either in hydrogen bonding to the ligands or in the maintenance of the hydrophilic sheath around the ligand	700352	
3.2.1.25	Y537A	low activity	680752