3.2.1.165	D139A	the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme. The mutant enzyme exclusively exhibits glycosynthase activity using alpha-D-glucosaminyl fluoride and D-glucosamine as substrates	-, 749852	
3.2.1.165	D139E	compared with the wild type, the hydrolytic activity of the mutant is significantly suppressed (less than 0.1%), and the fluoride release activity also decreases to less than 3%	-, 749852	
3.2.1.165	D139S	the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme	-, 749852	
3.2.1.165	D143A	the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme	-, 749852	
3.2.1.165	D178N	inactive	751103	
3.2.1.165	D180N	inactive	751103	
3.2.1.165	D464A	specific acitivity is 0.44% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	D464E	no activity	748421	
3.2.1.165	D464E/E539D	no activity	748421	
3.2.1.165	D464N	specific acitivity is 1.9% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	D469A	catalytic acid/base	699562	
3.2.1.165	D469A	inactive mutant enzyme	680031, 680406	
3.2.1.165	D469E	inactive mutant enzyme	680031, 680406	
3.2.1.165	D513N	specific acitivity is 72% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	D655N	specific acitivity is 103% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	E179Q	the mutant shows 3% of wild type activity	751103	
3.2.1.165	E181Q	the mutant shows 10% of wild type activity	751103	
3.2.1.165	E181Q	the mutant shows 15% of wild type activity	751103	
3.2.1.165	E306Q	the mutant shows 40% of wild type activity	751103	
3.2.1.165	E347Q	inactive	751103	
3.2.1.165	E349Q	inactive	751103	
3.2.1.165	E409Q	specific acitivity is 77% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	E415Q	specific acitivity is 53% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	E470Q	specific acitivity is 99% compared to the activity of the wild-type enzyme	748421	
3.2.1.165	E539A	no activity	748421	
3.2.1.165	E539D	no activity	748421	
3.2.1.165	E539Q	no activity	748421	
3.2.1.165	E541A	catalytic nucleophile	699562	
3.2.1.165	E541D	inactive mutant enzyme	680031, 680406	
3.2.1.165	E541Q	inactive mutant enzyme	680031, 680406	
3.2.1.165	E555A	the mutation significantly decreases the hydrolytic activity compared to the wild type enzyme	-, 749852	
3.2.1.165	F769W	the mutation significantly enhances the specific activity and hydrolysis velocity compared to the wild type enzyme	-, 751306	
3.2.1.165	S468N/D469E	inactive mutant enzyme	680031, 680406