3.2.1.140	D320A	site-directed mutagenesis, the mutant shows significantly reduced kcat, but unaffected Km compared with the wild-type enzyme	732092	
3.2.1.140	D320N	site-directed mutagenesis, the mutant shows significantly reduced kcat, but unaffected Km compared with the wild-type enzyme	732092	
3.2.1.140	H263F	site-directed mutagenesis, the mutant shows significantly reduced kcat compared with the wild-type enzyme	732092	
3.2.1.140	additional information	generation of different truncated forms of the enzyme, structure-function analysis in comparison to the wild-type, overview. The large Bifidobacterium bifidum lacto-N-biosidase and its truncated mutants are used as a model proteins to evaluate the minimal functional unit due to its interest and structural complexity	755088	
3.2.1.140	additional information	truncated enzyme without N-terminal signal peptide of 34 amino acids and C-terminal membrane anchor from 1065-1108	695742	
3.2.1.140	Y419F	site-directed mutagenesis, the mutant shows significantly reduced Km and kcat values compared with the wild-type enzyme	732092