3.1.4.54 367stop complete loss of activity 680640 3.1.4.54 377stop complete loss of activity 680640 3.1.4.54 387stop Vmax is 20% of wild-type activity, slight decrease in Km-value for N-palmitoyl-1,2-dioleoyl-phosphatidylethanolamine 680640 3.1.4.54 C170S Vmax is 44% of wild-type activity 680640 3.1.4.54 C222S Vmax is 88% of wild-type activity 680640 3.1.4.54 C224S considerable reduction in activity 708079 3.1.4.54 C224S Vmax is 7.8% of wild-type activity 680640 3.1.4.54 C237S Vmax is 90% of wild-type activity 680640 3.1.4.54 C255S Vmax is 88% of wild-type activity 680640 3.1.4.54 C288S Vmax is 87% of wild-type activity 680640 3.1.4.54 D284N complete loss of activity 680640 3.1.4.54 D389N Vmax is about 87% of wild-type activity, single nucleotide polymorphism mutant 680640 3.1.4.54 DELTAN138 complete loss of activity 680640 3.1.4.54 DELTAN55 Vmax is 28% of wild-type activity, slight decrease in Km-value for N-palmitoyl-1,2-dioleoyl-phosphatidylethanolamine 680640 3.1.4.54 DELTAN85 complete loss of activity 680640 3.1.4.54 H185N complete loss of activity 680640 3.1.4.54 H187N complete loss of activity 680640 3.1.4.54 H189N complete loss of activity 680640 3.1.4.54 H190N complete loss of activity 680640 3.1.4.54 H253N complete loss of activity 680640 3.1.4.54 H321N complete loss of activity 680640 3.1.4.54 H331N specific activity is about 4% of wild-type value 680640 3.1.4.54 H343N specific activity is similar to wild-type value 680640 3.1.4.54 H353N specific activity is similar to wild-type value 680640 3.1.4.54 H380R complete loss of activity, single nucleotide polymorphism mutant 680640 3.1.4.54 H380R considerable reduction in activity 708079 3.1.4.54 H380R Vmax is about 4% of wild-type activity, single nucleotide polymorphism mutant 680640 3.1.4.54 L207F complete loss of activity, single nucleotide polymorphism mutant of 680640 3.1.4.54 L207F considerable reduction in activity 708079 3.1.4.54 L207F Vmax is about 1% of wild-type activity, single nucleotide polymorphism mutant 680640 3.1.4.54 additional information construction of and food intake studies in NAPE-PLD-/- mice, phenotype, overview -, 714338 3.1.4.54 additional information generation of NAPE-PLD-/- mice, phenotype, overview -, 714339 3.1.4.54 Q158S/Y159S mutant is unable to form stable dimers and shows a decreased melting temperature 752275 3.1.4.54 Q320A decrease in catalytic activity 750295 3.1.4.54 Q320S decrease in catalytic activity 750295 3.1.4.54 S152A Vmax is about 84% of wild-type activity, single nucleotide polymorphism mutant 680640