3.1.2.12	C26A	site-directed mutagenesis, the YeiG mutant shows wild-type activity	-, 665750	
3.1.2.12	C54A	site-directed mutagenesis, the YeiG mutant shows wild-type activity	-, 665750	
3.1.2.12	C54S	shows a 4fold decrease in alpha-naphthyl butyrate activity. Is insensitive to the inhibitor N-ethylmaleimide	710495	
3.1.2.12	C59S	mutant enzyme is highly active in hydrolysis of 4 -methylumbelliferyl acetate	682315	
3.1.2.12	C59S	site-directed mutagenesis, the mutant shows no hydrolase activity with S-formylglutathione, but with 4-methylumbelliferyl acetate in contrast to the wild-type enzyme	666081	
3.1.2.12	C60A	Km and kcat(4-nitrophenyl acetate) slightly decreased compared to wild-type	731220	
3.1.2.12	C60H/W197I	mutant shows significantly decreased activity	691005	
3.1.2.12	C60K/W197I	the mutation results in a further enhancement of the rates of phosphorylation with paraoxon but does not affect the dephosphorylation of the enzyme	691005	
3.1.2.12	C60Q/W197I	mutant shows significantly decreased activity	691005	
3.1.2.12	C60R/W197I	the mutation results in a further enhancement of the rates of phosphorylation with paraoxon but does not affect the dephosphorylation of the enzyme	691005	
3.1.2.12	C60S	Km and kcat(4-nitrophenyl acetate) slightly decreased compared to wild-type. mutant is not inactivated by peroxide	731220	
3.1.2.12	C60S	mutant shows significantly decreased activity	691005	
3.1.2.12	C60S/W197I	mutant shows significantly decreased activity	691005	
3.1.2.12	D199A	site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme	-, 665750	
3.1.2.12	D218A	site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme	-, 665750	
3.1.2.12	D223A	site-directed mutagenesis, the YeiG mutant shows no esterase activity	665750	
3.1.2.12	D255A	site-directed mutagenesis, the YeiG mutant shows 2fold reduced activity compared to the wild-type enzyme	665750	
3.1.2.12	D80A	site-directed mutagenesis, the YeiG mutant shows reduced activity compared to the wild-type enzyme	665750	
3.1.2.12	G57H	mutant shows significantly decreased activity	691005	
3.1.2.12	H160I	Oxidation leads to activation of mutant enzyme. Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Oxidized form (with or without catalase): Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) increased compared to wild-type	731220	
3.1.2.12	H160S	Km (4-nitrophenyl acetate) slightly decreased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type	731220	
3.1.2.12	L58H/W197I	mutant shows significantly decreased activity	691005	
3.1.2.12	M162H	mutant shows significantly decreased activity	691005	
3.1.2.12	M162H/C60S/W197I	mutant shows significantly decreased activity	691005	
3.1.2.12	additional information	construction of FrmB and YeiG deletion mutants	-, 665750	
3.1.2.12	additional information	fgh1 gene disruption impairs the ability to grow on methanol and reduces the growth rate on methylamine and choline as sole nitrogen source, while growth on glucose or glycerol is not affected	653164	
3.1.2.12	N64A	Km (4-nitrophenyl acetate) slightly increased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type	731220	
3.1.2.12	S147A	is unable to hydrolyse alpha-naphthyl butyrate	710495	
3.1.2.12	S152A	site-directed mutagenesis, completely inactive mutant	666081	
3.1.2.12	S154A	site-directed mutagenesis, the YeiG mutant shows no esterase activity	665750	
3.1.2.12	W197I	Km (4-nitrophenyl acetate) decreased and kcat (4-nitrophenyl acetate) decreased compared to wild-type	731220	
3.1.2.12	W197I	the substitution enhances SFGH reactivity with paraoxon by more than 1000fold thereby overcoming natural organophosphate resistance, the mutant increases the rate of organophosphate inhibition under pseudo-first-order conditions but does not accelerate organophosphate hydrolysis	691005	
3.1.2.12	W197I/C60S	Km (4-nitrophenyl acetate) decreased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Km (S-lactolylglutathione) increased and kcat (4-nitrophenyl acetate) highly decreased compared to wild-type	731220	
3.1.2.12	W197I/H160I	Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Km (S-lactolylglutathione) decreased and kcat (4-nitrophenyl acetate) highly decreased compared to wild-type	731220	
3.1.2.12	Y278F	Km (4-nitrophenyl acetate) slightly decreased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type	731220