2.7.8.43 D463A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 E244A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 H358A site-directed mutagenesis, mutation of a potential zinc binding residue -, 739786 2.7.8.43 H393A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 H440A site-directed mutagenesis, mutation of a potential zinc binding residue -, 739786 2.7.8.43 H464A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 H476A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 I36R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 I36R1/Q148A/K149A site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 I86R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 I86R1/Q148A/K149A site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 L145R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 L45R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 M98R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 additional information construction of a leptA knockout mtant -, 738368 2.7.8.43 additional information construction of a membrane-deletion mutant of the enzyme -, 733017 2.7.8.43 additional information construction of a pmrA- null mutant strain JSG421 of covalently modified pmrAc strains JSG485 and JSG486, overview -, 734138 2.7.8.43 additional information construction of Cronobacter sakazakii mutant strains WLL001, a ESA_RS09200 deletion mutant, and of WLL002, WLL003 and WLL004, with mutationsof genes lpxE, ESA-RS16430, and ESA_RS16425, with or without additional deletion of ESA_RS09200. Analysis of mammalian cell invasion abilities of mutants using human enterocyte-like epithelial Caco-2 cells, overview -, 738476 2.7.8.43 additional information construction of gene lptA mutant and complement strains from strain FA 1090 -, 733635 2.7.8.43 additional information construction of insertional lptA mutants from 35E (L2), H44/76 (L3), and 89I (L4) backgrounds 734062 2.7.8.43 additional information construction of several enzyme mutant strains, overview -, 733966 2.7.8.43 additional information construction of the opgE::cml mutant, and of the opgE::Tn5 mutant by random transposon mutagenesis with Tn5 740134 2.7.8.43 additional information domain swapping between isoforms Mcr1 and Mcr2 shows that the presence of the resultant two chimeric genes can confer resistance of the colistin-susceptible strain MG1655 to up to 16 mg/liter of colistin 761797 2.7.8.43 additional information generation of a mutant in Campylobacter jejuni 81-176 by interruption of cj0256 results in the absence of pEtN modifications on lipid A as well as FlgG 735157 2.7.8.43 additional information generation of a soluble enzyme mutant by deletion of the membrane binding structure. The mutant does not confer resistance to polymyxin in transformed Escherichia coli cells, in contrast to the wild-type LptA -, 734476 2.7.8.43 additional information generation of an lptA deletion mutant strain using plasmid pMEB256, with or without combined deletions of genes ptdA and ptdB 735018 2.7.8.43 additional information generation of polymyxin-sensitive mutants of an Escherichia coli pmrAC strain WD101 producing a lipid A that lacked both the 3'-acyloxyacyl-linked myristate (C14) and L-4-aminoarabinose, even though the necessary enzymatic machinery required to synthesize L-4-aminoarabinose-modified lipid A is present. Strain WD103 is generated by P1vir transduction of the polymyxin-resistant genotype (pmrAC) of strain WD101 into the Escherichia coli lpxM mutant MLK1067, phenotype, overview. The polymyxin-sensitive parent strains MLK1067 and W3110 produce unmodified penta- or hexa-acylated lipid A, respectively. Complementation of WD103 with the vector pWSLpxM resulted in restoration of polymyxin resistance to the levels displayed by WD101, effect of lipid myristoylation on the polymyxin resistance 734151 2.7.8.43 additional information JSG435 carries a mutant pmrA locus allele (pmrA505), JSG435 is mutagenized with the transposon Tn10d. Chromosomal mapping of the transposon insertions of the two polymyxin B sensitive strains shows them to be located at 50-52 centisomes (JSG485) and 43-45 centisomes (JSG486), thus eliminating the possibility that mutations in these regulatory loci are responsible for the observed phenotypic change -, 734706 2.7.8.43 additional information lipid A prepared from the PA14DELTAeptAPa mutant shows no phosphatidylethanolamine modification when Zn2+ is added to the media 739106 2.7.8.43 additional information neisserial LptA stability is dependent on the presence of an oxidoreductase DsbA, LptA::Hisx6 expressed in JCB571 (CKEC543), in which the chromosomal copy of dsbA has been insertionally inactivated, is not stable and is rapidly removed via proteolytic degradation -, 739495 2.7.8.43 additional information piliated colony variants of strain FA19 are transformed with plasmid or chromosomal DNA preparations bearing insertionally inactivated lpt3, lpt6, or lptA genes prepared previously from Neisseria meningitidis strain NMB -, 733963 2.7.8.43 N185R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 N185R1/Q148A/K149A site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 N308A site-directed mutagenesis, mutation of a potential zinc binding residue -, 739786 2.7.8.43 Q148A/K149A site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 S110R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 S309A site-directed mutagenesis, mutation of a potential zinc binding residue -, 739786 2.7.8.43 T266A site-directed mutagenesis, mutation of a potential zinc binding residue 739786 2.7.8.43 T266S site-directed mutagenesis, mutation of a potential zinc binding residue -, 739786 2.7.8.43 T283A mutant cannot confer robust growth to the recipient strain in presence of more than 2 mg/liter of colistin 761797 2.7.8.43 T315A mutation of the catalytic domain, abolishes antibiotic resistance activity 760257 2.7.8.43 T32R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 V132R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134 2.7.8.43 V165R1 site-directed mutagenesis, altered lipopolysaccharide binding kinetics compared to wild-type 752134