2.7.7.B5	C207S	using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis	725262	
2.7.7.B5	C344S	using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis	725262	
2.7.7.B5	C456S	using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis	725262	
2.7.7.B5	D189A	mutation eliminates in vivo function of the enzyme	691789	
2.7.7.B5	D189A	using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis	725262	
2.7.7.B5	K321M	mutation eliminates in vivo function of the enzyme	691789	
2.7.7.B5	K321M	using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis	725262	
2.7.7.B5	additional information	construction of two truncated forms of ThiI containing the N-terminal domain including NFLD and THUMP, and the C-terminal domain including the PP-loop and RLD domains, respectively. The N-domain can bind with both tRNAPhe and TPHE39A and recognizes the acceptor-stem region, whereas the C-domain cannot. The C-domain also affects RNA binding by its enthalpically favorable, but entropically unfavorable, contribution. The C-domain induces a conformation change in tRNAPhe	710548