2.7.7.14	H146A	site-directed mutagenesis, the mutant is almost inactive	721549	
2.7.7.14	H226A	mutation in histidine residue in the HxGH motif of the C-terminal CT domain. Decrease in activity	737597	
2.7.7.14	H229A	mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Activity similar to wild-type activity	737597	
2.7.7.14	H244A	inactive C-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity	738322	
2.7.7.14	H244Y	inactive C-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity	738322	
2.7.7.14	H35A	inactive N-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity	738322	
2.7.7.14	H35A	mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Complete loss of activity	737597	
2.7.7.14	H35Y	inactive N-domain mutant, presence of the mutant reduces splice variant Pcyt2alpha homodimerization and activity	738322	
2.7.7.14	H38A	mutation in histidine residue in the HxGH motif of the N-terminal CT domain. Strong decrease in activity	737597	
2.7.7.14	H422A	site-directed mutagenesis, the mutant shows increased kcat and Vmax with ethanolamine phosphate compared to the wild-type enzyme	721549