2.5.1.96	A726G, A850G	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	715351	
2.5.1.96	F26L	mutation is sufficient to gain phytoene synthase activity, decrease in dehydrosqualene synthase activity	-, 715351	
2.5.1.96	F26L/E149G	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	-, 715351	
2.5.1.96	F26L/F267S	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	715351	
2.5.1.96	F26L/V43M	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	-, 715351	
2.5.1.96	F26S/I40T	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	715351	
2.5.1.96	G856A/G1125A/T1128C/T1176C	directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation of gene ysqs	738235	
2.5.1.96	H12R/F26L/D27G/90G/K97R/H207R	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	715351	
2.5.1.96	K20E/F26S	mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity	-, 715351	
2.5.1.96	M4V/H12R/F59S/Q81R/E180G	mutant with phytoene synthase activity, without decrease in dehydrosqualene synthase activity	715351	
2.5.1.96	additional information	construction of C40 carotenoid synthase pathway in Escherichia coli by expression of crtE encoding GGPP synthase, crtB encoding phytoene synthase, and crtI encoding phytoene desaturase, all from Erwinia uredovora. Replacement of phytoene synthase crtB by dehydrosqualene synthase crtM results in negligible ability to synthesize the C40 product. Some crtM mutants perform comparably to crtB in an in vivo C40 pathway. These mutants show significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity	-, 715351	
2.5.1.96	additional information	directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation gene tsqs replacement with the human gene mutant Q33R/D34N/S38N/G856A/G1125A/T1128C/T1176C	738235	
2.5.1.96	additional information	expression of CrtM in Escherichia coli leads to synthesis of diapophytoene from pre-existing farnesyl diphosphate	-, 713989	
2.5.1.96	Q33R/D34N/S38N	directed evolution of human Q33R/D34N/S38N mutant squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by replacement of hsqs in pAC-hsqs with the Saccharomyces mutant gene ysqs G856A/G1125A/T1128C/T1176C	738235	
2.5.1.96	Y129A	no activity with substrate farnesyl diphophate, 7% residual activity with geranyl diphosphate	716765	
2.5.1.96	Y248A	crystallization data of complex with zaragozic acid A	722759