2.5.1.79	E156D	besides main product thermospermine, mutant is able to synthesize some spermine	
2.5.1.79	additional information	contrary to wild-type, seedlings of acl5-1 loss-of-function mutant do not contain thermospermine. Daily application of thermospermine onto the shoot apex partially rescues the dwarf phenotype of the mutant, while application of spermine has no effect. The acl5-1 transcript level in acl5-1 seedlings, which is much higher than the ACL5 transcript level in wild-type seedlings, is reduced by exogenous thermospermine	
2.5.1.79	additional information	constitutive expression of the cotton ACL5 gene (GhACL5) in transgenic Arabidopsis thaliana ecotype Columbia-0, via Agrobacterium tumefaciens strain GV3101 transfection using the CaMV 35S promoter, significantly increases plant height and elevates the level of thermospermidine, quantitative real-time RT-PCR expression analysis of transgenic GhACL5 and endogenous AtACL5 transcripts, transcript levels of endogenous AtACL5 are reduced in plants that ectopically express gene GhACL5, seedling phenotypes, overview. Germination rate for seeds from GhACL5-overexpressing plants is lower when compared with control after 3 days	
2.5.1.79	additional information	enzme overexpression under the control of the 35S CaMV constitutive promoter in Populus tremula x Populus tremuloides plants leads to reduced overall growth and slight defects in xylem development. Ectopic expression of POPACAULIS5 results in the dramatic changes in shoot and root development, and the transgenic lines shows (severe) dwarf phenotypes, overview. Some dwarf transgenic plants are able to develop a rooting system and show partial restoration of the wild-type phenotype once transferred to a plant growth regulator-free medium. Thermospermine accumulation is suppressed in the 35S::POPACAULIS5 Populus woody stem, but not in leaves. Indole-3-acetic acid levels are strongly reduced in the leaves and in the secondary xylem tissues of the transgenic trees	
2.5.1.79	additional information	generation of GhACL5-sielenced plants endogenous levels of putrescine, spermidine, and spermine are similar between GhACL5-silenced and control plants. GhACL5-silenced plants show a severe dwarf phenotype