2.5.1.27	D173G	decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 208 compared with 2.3 for wild-type	689799	
2.5.1.27	D49A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	E210N	slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 15 compared with 2.3 for wild-type	689799	
2.5.1.27	E213Q	slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 5.1 compared with 2.3 for wild-type	689799	
2.5.1.27	F120A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	F157A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	F93A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	H214L	decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 1163 compared with 2.3 for wild-type	689799	
2.5.1.27	additional information	ectopic expression of isoform IPT2 in calli and in planta of Arabidopsis thaliana creates phenotypes consistent with cytokinin overproduction	689564	
2.5.1.27	additional information	generation of SAG12-IPT transgenic cassava, analysis of the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system, phenotypes overview	722898	
2.5.1.27	additional information	regulated expression of IPT in peanut significantly improves drought tolerance in both laboratory and field conditions, transgenic peanut plants produce significantly higher yields than wild-type control plants in the field, phenotypes, overview	723389	
2.5.1.27	additional information	transgenic expression in Nicotiana tabacum increases cytokinin levels and promotes cell division in the mebryo in transgenic seeds. Number of plerome cell layers and the cell number of cotyledons and pleromes are significantly increased from 16 days after flowering, the embryo diameter of transgenic tobacco is enlarged. The soluble protein content of the transgenic mature seeds is increased by 9.8-22.2% and the dry weight of transgenic tobacco seeds is increased by 8.8-21.8% compared with that of controls	688633	
2.5.1.27	Q255A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	W159A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	Y153A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	Y170A	retains 84% activity compared to the wild type enzyme	671796	
2.5.1.27	Y211T	slight decrease in activity with substrate 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. Specificity ratio for substrate dimethylallyl diphosphate over 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate is 9 compared with 2.3 for wild-type	689799	
2.5.1.27	Y217A	almost complete loss of activity compared to the wild type enzyme	671796	
2.5.1.27	Y54A	almost complete loss of activity compared to the wild type enzyme	671796