2.4.1.288	D256A	the mutant shows almost no activity even in the presence of Mn2+ or Mg2+	-, 718920	
2.4.1.288	D256E	the mutant shows almost no activity even in the presence of Mn2+ or Mg2+	-, 718920	
2.4.1.288	D258A	the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+	-, 718920	
2.4.1.288	D258E	the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme	-, 718920	
2.4.1.288	D371A	inactive	-, 718920	
2.4.1.288	D372A	inactive	718920	
2.4.1.288	D372E	inactive	718920	
2.4.1.288	D372S	the mutant shows no detectable activity	720037	
2.4.1.288	E300S	the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme	720037	
2.4.1.288	E371S	the mutant shows no detectable activity	720037	
2.4.1.288	H413S	the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme	720037	
2.4.1.288	W399S	the mutant exhibits increased Km and strongly decreased kcat values compare to the wild type enzyme	720037