2.3.1.282 A382F almost complete loss of activity -, 749897 2.3.1.282 A382M 35% of wild-type activity with substrate 1-octanol, 39% with 5R,7R-undecane-5,7-diol, 48% with pentadecane-5,7-diol, respectively -, 749897 2.3.1.282 D128A mutation in HHx3DGx13Y motif, loss of activity -, 752024 2.3.1.282 G129L 13% of wild-type activity with substrate 1-octanol, 4% with 5R,7R-undecane-5,7-diol, 13% with pentadecane-5,7-diol, respectively -, 749897 2.3.1.282 H123A about 1.7- to 2.3fold decrease in ester formation -, 752024 2.3.1.282 H124A mutation in HHx3DGx13Y motif, loss of activity -, 752024 2.3.1.282 Q19K 16% of wild-type activity with substrate 1-octanol, 11% with pentadecane-5,7-diol, respectively 749897 2.3.1.282 Q19R 68% of wild-type activity with substrate 1-octanol, 48% with 5R,7R-undecane-5,7-diol, 45% with pentadecane-5,7-diol, respectively -, 749897 2.3.1.282 S380M almost complete loss of activity -, 749897 2.3.1.282 V16C/G328C 8% of wild-type activity with with 5R,7R-undecane-5,7-diol, 3% with pentadecane-5,7-diol, respectively 749897 2.3.1.282 Y143F about 1.9fold decrease in ester formation -, 752024