2.3.1.258	F27A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	F27A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	F35A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	F35A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	H112A	inactive	720002	
2.3.1.258	H112A	NMR spectroscopy using the catalytically inactive hNaa50p mutant	720002	
2.3.1.258	H112A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	H112A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	H112F	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	H112F	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	I142A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	719976	
2.3.1.258	I142A	the mutant shows 42.2% of wild type catalytic efficiency	719976	
2.3.1.258	L814P	site-directed mutagenesis, the hNAA15 mutant is defective for HYPK inhibition and reduces hNatA thermostability, hNAA10 binding is not affected	757754	
2.3.1.258	additional information	generation of mutants MtRimI4-158, MtRimI1-153, MtRimI4-153, MtRimIC21A, and of the final construct MtRimIC21A4-153, MtRimIC21A4-153 has almost identical enzymatic activity compared to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. The 2D 1H-15N heteronuclear single quantum coherence spectrum of tRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments show that MtRimIC21A4-153 possesses similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Structure comparison of wild-type MtRimI and mutant MtRimIC21A4-153	-, 755712	
2.3.1.258	additional information	N-terminal analyses comparing wild-type and scNaa50 deletion strains of Saccharomyces cerevisiae	-, 758492	
2.3.1.258	additional information	recombinant GST-tagged hNaa50 fails to pull down Schizosaccharomyces pombe SpNatA and hNaa50 and SpNatA cannot form a stoichiometric complex	-, 758493	
2.3.1.258	P28A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	719976	
2.3.1.258	P28A	the mutant shows less 13.4% of wild type catalytic efficiency	719976	
2.3.1.258	T406Y	site-directed mutagenesis, the hNAA15 mutant can disassociate hNAA50 from hNatA in vitro, hNAA10 binding is not affected	757754	
2.3.1.258	V29A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	719976	
2.3.1.258	V29A	the mutant shows 12.3% of wild type catalytic efficiency	719976	
2.3.1.258	Y124F	the mutant shows decreased activity compared to the wild type enzyme	740792	
2.3.1.258	Y139A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	Y139A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	Y31A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	Y31A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	Y73A	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	Y73A	the mutant shows less than 10% of wild type catalytic efficiency	719976	
2.3.1.258	Y73F	site-directed mutagenesis, inactive mutant	719976	
2.3.1.258	Y73F	the mutant shows less than 10% of wild type catalytic efficiency	719976