2.3.1.221 D1964N site-directed mutagenesis of a catalytic residue 723638 2.3.1.221 D2070N site-directed mutagenesis, the mutation results in only a slightly reduced rate of hydrolysis compared to the apo-mutant 723638 2.3.1.221 H2088F site-directed mutagenesis of a catalytic residue 723638 2.3.1.221 additional information construction of strain SRRC 2043, a disruption mutant defective in the pksA locus -, 723194 2.3.1.221 S1937A inactive mutant 722459 2.3.1.221 S1937A site-directed mutagenesis of a catalytic residue 723638