2.1.1.41	A193L	no activity	685376	
2.1.1.41	A193L	no enzyme activity	685376	
2.1.1.41	A193S	95% relative enzyme activity	685376	
2.1.1.41	A193S	relative activity: 95%	685376	
2.1.1.41	A196L	73% relative enzyme activity	685376	
2.1.1.41	A196L	relative activity: 73%	685376	
2.1.1.41	A200L	4% relative enzyme activity	685376	
2.1.1.41	A200L	relative activity: 4%	685376	
2.1.1.41	A221L	25% relative enzyme activity	685376	
2.1.1.41	A221L	relative activity: 25%	685376	
2.1.1.41	A350L	100% relative enzyme activity	685376	
2.1.1.41	A350L	relative activity: 100%	685376	
2.1.1.41	C128L	no activity	685376	
2.1.1.41	C128L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.028, photolabelled: value below 1%	688773	
2.1.1.41	C128L	no enzyme activity	685376	
2.1.1.41	C128S	Km (mM) (substrate: AdoMet): 30, kcat (1/sec) (substrate: AdoMet): 0.005, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.008	688773	
2.1.1.41	C198L	95% relative enzyme activity	685376	
2.1.1.41	C198L	relative activity: 95%	685376	
2.1.1.41	C198V	16% relative enzyme activity	685376	
2.1.1.41	C198V	relative activity: 16%	685376	
2.1.1.41	D125L	inactive mutant	658072	
2.1.1.41	D125L	no activity	685376	
2.1.1.41	D125L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.006, Kd (mM) (AdoMet): 0.012, photolabelled: 30%	688773	
2.1.1.41	D152L	inactive mutant	658072	
2.1.1.41	D152L	no activity	685376	
2.1.1.41	D152L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.005, Kd (mM) (AdoMet): 0.014, photolabelled: 35%	688773	
2.1.1.41	D189L	relative activity: 91%	685376	
2.1.1.41	D189L	the ratio of turnover-number to KM-value for zymosterol is 1.4fold lower than the wild-type ratio	658072	
2.1.1.41	D229L	relative activity: 100%	685376	
2.1.1.41	D276L	inactive mutant	658072	
2.1.1.41	D276L	no activity	685376	
2.1.1.41	D65L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	485340	
2.1.1.41	D65L	relative activity: 100%	685376	
2.1.1.41	D79L	potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol	485340	
2.1.1.41	D79L	relative activity: 81%	685376	
2.1.1.41	E108L	relative activity: 34%	685376	
2.1.1.41	E108L	the ratio of turnover-number to KM-value for zymosterol is 11fold lower than the wild-type ratio	658072	
2.1.1.41	E195L	inactive mutant	658072	
2.1.1.41	E195L	no activity	685376	
2.1.1.41	E209L	relative activity: 79%	685376	
2.1.1.41	E209L	the ratio of turnover-number to KM-value for zymosterol is 2.4fold lower than the wild-type ratio	658072	
2.1.1.41	E224L	relative activity: 74%	685376	
2.1.1.41	E246L	relative activity: 6%	685376	
2.1.1.41	E246L	the ratio of turnover-number to KM-value for zymosterol is 13.5fold lower than the wild-type ratio	658072	
2.1.1.41	E64L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	485340	
2.1.1.41	E64L	relative activity: 100%	685376	
2.1.1.41	E68L	no activity	685376	
2.1.1.41	E68L	potentially responsible for substrate binding to leucine by site directed mutagenesis, no activity	485340	
2.1.1.41	E82D	55% relative enzyme activity	685376	
2.1.1.41	E82D	relative activity: 55%	685376	
2.1.1.41	E82L	potentially responsible for substrate binding to leucine by site directed mutagenesis, not decreased activity, 1 new activity to form 24-methylzymosterol from zymosterol	485340	
2.1.1.41	E82L	produces plant-like substrate-product profiles	671933	
2.1.1.41	E82L	relative activity: 99%	685376	
2.1.1.41	E82Q	90% relative enzyme activity	685376	
2.1.1.41	E82Q	relative activity: 90%	685376	
2.1.1.41	E98L	potentially responsible for substrate binding to leucine by site directed mutagenesis, unchanged activity	485340	
2.1.1.41	E98L	relative activity: 100%	685376	
2.1.1.41	F178L	34% relative enzyme activity	685376	
2.1.1.41	F178L	relative activity: 34%	685376	
2.1.1.41	F183L	100% relative enzyme activity	685376	
2.1.1.41	F188L	2% relative enzyme activity	685376	
2.1.1.41	F188L	relative activity: 2%	685376	
2.1.1.41	F220L	29% relative enzyme activity	685376	
2.1.1.41	F220L	relative activity: 29%	685376	
2.1.1.41	F269L	no activity	685376	
2.1.1.41	F269L	no enzyme activity	685376	
2.1.1.41	F357L	100% relative enzyme activity	685376	
2.1.1.41	F357L	relative activity: 100%	685376	
2.1.1.41	F89L	100% relative enzyme activity	685376	
2.1.1.41	F89L	relative activity: 100%	685376	
2.1.1.41	F91L	15% relative enzyme activity	685376	
2.1.1.41	F91L	relative activity: 15%	685376	
2.1.1.41	G127L	1% relative enzyme activity	685376	
2.1.1.41	G127L	relative activity: 1%	685376	
2.1.1.41	G129L	no activity	685376	
2.1.1.41	G129L	no enzyme activity	685376	
2.1.1.41	G131L	5% relative enzyme activity	685376	
2.1.1.41	G131L	relative activity: 5%	685376	
2.1.1.41	G132L	50% relative enzyme activity	685376	
2.1.1.41	G132L	relative activity: 50%	685376	
2.1.1.41	G217L	100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols	685376	
2.1.1.41	G217L	relative activity: 100%	685376	
2.1.1.41	G218L	100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols	685376	
2.1.1.41	G218L	relative activity: 100%	685376	
2.1.1.41	G347L	5% relative enzyme activity	685376	
2.1.1.41	G347L	relative activity: 5%	685376	
2.1.1.41	G351L	90% relative enzyme activity	685376	
2.1.1.41	G351L	relative activity: 90%	685376	
2.1.1.41	G352L	3% relative enzyme activity	685376	
2.1.1.41	G352L	relative activity: 3%	685376	
2.1.1.41	G84L	14% relative enzyme activity	685376	
2.1.1.41	G84L	relative activity: 14%	685376	
2.1.1.41	G86L	16% relative enzyme activity	685376	
2.1.1.41	G86L	relative activity: 16%	685376	
2.1.1.41	H107L	relative activity: 23%	685376	
2.1.1.41	H107L	the ratio of turnover-number to KM-value for zymosterol is 2.4fold lower than the wild-type ratio	658072	
2.1.1.41	H199L	relative activity: 2%	685376	
2.1.1.41	H199L	the ratio of turnover-number to KM-value for zymosterol is 162fold lower than the wild-type ratio	658072	
2.1.1.41	H199Q	45% relative enzyme activity	685376	
2.1.1.41	H199Q	relative activity: 45%	685376	
2.1.1.41	H199R	20% relative enzyme activity	685376	
2.1.1.41	H199R	relative activity: 20%	685376	
2.1.1.41	H224L	the ratio of turnover-number to KM-value for zymosterol is 1.4fold lower than the wild-type ratio	658072	
2.1.1.41	H238L	relative activity: 85%	685376	
2.1.1.41	H238L	the ratio of turnover-number to KM-value for zymosterol is 1.6fold lower than the wild-type ratio	658072	
2.1.1.41	H90L	inactive mutant	658072	
2.1.1.41	H90L	no activity	685376	
2.1.1.41	H90Q	no activity	685376	
2.1.1.41	H90Q	no enzyme activity	685376	
2.1.1.41	H90R	no activity	685376	
2.1.1.41	H90R	no enzyme activity	685376	
2.1.1.41	I194L	67% relative enzyme activity	685376	
2.1.1.41	I194L	relative activity: 67%	685376	
2.1.1.41	K215L	100% relative enzyme activity	685376	
2.1.1.41	K215L	relative activity: 100%	685376	
2.1.1.41	K353L	56% relative enzyme activity	685376	
2.1.1.41	additional information	by photoaffinity labeling and mutational analysis the AdoMet binding site is defined. Results indicate that one or both of Cys128 and Pro133 are covalently bound to AdoMet	688773	
2.1.1.41	additional information	C57BL/6 mice immunized with a vaccine candidate consisting of 24-c-methyltransferase as an antigene formulated monophosphoryl lipid A (MPL-SE) show Ag-specific Th1 immune responses characterized by robust production of IFN-gamma upon specific Ag re-exposure in vitro. Upon challenge with Leishmania infantum, mice immunized with SMT plus MPL-SE show significant lower parasite burdens in both spleens and livers compared with non-immunized mice or mice injected with adjuvant alone	690178	
2.1.1.41	additional information	Leishmania major promastigotes cells overproducing the enzyme do not have increased resistance to the sterol methenyltransferase inhibitor 22,26-azasterol	688896	
2.1.1.41	additional information	natural amphotericine B-resistant mutant strain	485329	
2.1.1.41	additional information	scanning mutagenesis experiments involving a leucine replacement of 52 amino acids in Erg6p followed by substitution of key residues with functionally or structurally similar amino acids indicate that 5 new residues at positions Y192, G217, G218, T219 and Y223 can switch the course of C1-transfer activity to include plant-like C2-transfer activity. The data support a model in which several conserved and non-conserved amino acids located in distinct regions of the Saccharomyces cerevisiae Erg6p regulate the course of the C-methylation reaction toward product differences	685376	
2.1.1.41	P133L	no activity	685376	
2.1.1.41	P133L	no activity with AdoMet as substrate, Kd (mM) (zymosterol): 0.015, Kd (mM) (AdoMet): no binding, photolabelled: value below 1%	688773	
2.1.1.41	P133L	no enzyme activity	685376	
2.1.1.41	P201L	58% relative enzyme activity	685376	
2.1.1.41	P201L	relative activity: 58%	685376	
2.1.1.41	P216L	65% relative enzyme activity	685376	
2.1.1.41	P216L	relative activity: 65%	685376	
2.1.1.41	S354L	100% relative enzyme activity	685376	
2.1.1.41	S354L	relative activity: 100%	685376	
2.1.1.41	S87E	100% relative enzyme activity	685376	
2.1.1.41	S87L	100% relative enzyme activity	685376	
2.1.1.41	S87L	relative activity: 100%	685376	
2.1.1.41	S87Q	100% relative enzyme activity	685376	
2.1.1.41	S88L	61% relative enzyme activity	685376	
2.1.1.41	S88L	relative activity: 61%	685376	
2.1.1.41	S88N	17% relative enzyme activity	685376	
2.1.1.41	S88N	relative activity: 17%	685376	
2.1.1.41	S97E	relative activity: 100%	685376	
2.1.1.41	S97Q	relative activity: 100%	685376	
2.1.1.41	T197L	93% relative enzyme activity	685376	
2.1.1.41	T197L	relative activity: 93%	685376	
2.1.1.41	T219L	32% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols	685376	
2.1.1.41	V126L	1% relative enzyme activity	685376	
2.1.1.41	V126L	relative activity: 1%	685376	
2.1.1.41	V130L	100% relative enzyme activity	685376	
2.1.1.41	V130L	relative activity: 100%	685376	
2.1.1.41	V222L	27% relative enzyme activity	685376	
2.1.1.41	V349L	47% relative enzyme activity	685376	
2.1.1.41	V349L	relative activity: 47%	685376	
2.1.1.41	W225F	100% relative enzyme activity	685376	
2.1.1.41	W225F	relative activity: 60%	685376	
2.1.1.41	W225L	100% relative enzyme activity	685376	
2.1.1.41	W225L	relative activity: 100%	685376	
2.1.1.41	W286F	100% relative enzyme activity	685376	
2.1.1.41	W286F	relative activity: 51%	685376	
2.1.1.41	W286L	2% relative enzyme activity	685376	
2.1.1.41	W286L	relative activity: 2%	685376	
2.1.1.41	W85F	100% relative enzyme activity	685376	
2.1.1.41	W85F	relative activity: 55%	685376	
2.1.1.41	W85L	40% relative enzyme activity	685376	
2.1.1.41	W85L	relative activity: 40%	685376	
2.1.1.41	Y110F	no significant change in products	734863	
2.1.1.41	Y110L	remodeling the active site to alter the electronics results in delayed timing of the hydride migration from methyl attack of the DELTA24-bond, that thereby produces metabolic switching of product ratios in favor of DELTA25(27)-olefins or impairs the second C1-transfer activity	734863	
2.1.1.41	Y153F	100% relative enzyme activity	685376	
2.1.1.41	Y153F	Km (mM) (substrate: AdoMet): 0.016, kcat (1/sec) (substrate: AdoMet): 0.003, Kd (mM) (zymosterol): 0.007, Kd (mM) (AdoMet): 0.013, photolabelled: 55%	688773	
2.1.1.41	Y153F	relative activity: 41%	685376	
2.1.1.41	Y153L	Km (mM) (substrate: AdoMet): 0.017, kcat (1/sec) (substrate: AdoMet): 0.01, Kd (mM) (zymosterol): 0.004, Kd (mM) (AdoMet): 0.004	688773	
2.1.1.41	Y153L	no enzyme activity	685376	
2.1.1.41	Y153L	relative activity: 100%	685376	
2.1.1.41	Y177F	mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type	718790	
2.1.1.41	Y192F	100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols	685376	
2.1.1.41	Y192L	95% relative enzyme activity	685376	
2.1.1.41	Y207F	100% relative enzyme activity	685376	
2.1.1.41	Y207F	relative activity: 100%	685376	
2.1.1.41	Y207L	3% relative enzyme activity	685376	
2.1.1.41	Y207L	relative activity: 3%	685376	
2.1.1.41	Y208F	mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type	718790	
2.1.1.41	Y223F	100% relative enzyme activity, mutant can perform C2-transfer activity to give 24-ethyl(idene)-sterols	685376	
2.1.1.41	Y223F	relative activity: 100%	685376	
2.1.1.41	Y223L	100% relative enzyme activity	685376	
2.1.1.41	Y223L	relative activity: 100%	685376	
2.1.1.41	Y287L	20% relative enzyme activity	685376	
2.1.1.41	Y287L	relative activity: 20%	685376	
2.1.1.41	Y66F	mutant proteins catalyses zymosterol much less efficiently than the wild-type enzyme, with a Vmax/Km ratio 23-40% that of wild-type. Sterol composition of the Y66F catalysis is different. The sterol mixture possesses a significant decrease in ergosta-8,24(25)-dienol compensated by an increase in ergosta-8,25(27)-dienol and ergosta-8,24(28)-dienol	718790	
2.1.1.41	Y74F	100% relative enzyme activity	685376	
2.1.1.41	Y74F	relative activity: 100%	685376	
2.1.1.41	Y74L	100% relative enzyme activity	685376	
2.1.1.41	Y74L	relative activity: 100%	685376	
2.1.1.41	Y81A	Km (mM): 0.018 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.00083 (zymosterol), 0.000116 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00019 (25-azalanosterol), 0.00013 (24(R,S),25-epiminolanosterol), 0.071 (ergosterol)	684723	
2.1.1.41	Y81F	Km (mM): 0.017 (zymosterol), 0.026 (26,27-dehydrozymosterol), kcat (1/sec): 0.0108 (zymosterol), 0.0025 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00036 (25-azalanosterol), 0.00031 (24(R,S),25-epiminolanosterol), 0.082 (ergosterol)	684723	
2.1.1.41	Y81F	produces plant-like substrate-product profiles	671933	
2.1.1.41	Y81F	relative activity: 100%	685376	
2.1.1.41	Y81I	Km (mM): 0.021 (zymosterol), 0.027 (26,27-dehydrozymosterol), kcat (1/sec): 0.00433 (zymosterol), 0.00025 (26,27-dehydrozymosterol), Ki (mM): 0.009 (26,27-dehydrozymosterol), 0.00020 (25-azalanosterol), 0.00016 (24(R,S),25-epiminolanosterol), 0.077 (ergosterol)	684723	
2.1.1.41	Y81L	90% relative enzyme activity	685376	
2.1.1.41	Y81L	Km (mM): 0.018 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00416 (zymosterol), 0.00033 (26,27-dehydrozymosterol), Ki (mM): 0.011 (26,27-dehydrozymosterol), 0.00021 (25-azalanosterol), 0.00018 (24(R,S),25-epiminolanosterol), 0.078 (ergosterol)	684723	
2.1.1.41	Y81L	relative activity: 90%	685376	
2.1.1.41	Y81V	Km (mM): 0.026 (zymosterol), 0.024 (26,27-dehydrozymosterol), kcat (1/sec): 0.00116 (zymosterol), 0.00011 (26,27-dehydrozymosterol), Ki (mM): 0.007 (26,27-dehydrozymosterol), 0.000195 (25-azalanosterol), 0.000145 (24(R,S),25-epiminolanosterol), 0.074 (ergosterol)	684723	
2.1.1.41	Y81W	Km (mM): 0.017 (zymosterol), 0.023 (26,27-dehydrozymosterol), kcat (1/sec): 0.0133 (zymosterol), 0.0033 (26,27-dehydrozymosterol), Ki (mM): 0.010 (26,27-dehydrozymosterol), 0.00039 (25-azalanosterol), 0.00036 (24(R,S),25-epiminolanosterol), 0.088 (ergosterol)	684723	
2.1.1.41	Y83F	95% relative enzyme activity	685376	
2.1.1.41	Y83F	relative activity: 95%	685376	
2.1.1.41	Y83L	90% relative enzyme activity	685376	
2.1.1.41	Y83L	relative activity: 90%	685376	
2.1.1.41	Y83W	100% relative enzyme activity	685376	
2.1.1.41	Y83W	relative activity: 100%	685376