2.1.1.274	M156H	site-directed mutagenesis, the mutation of Met156 to His results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant	757984	
2.1.1.274	M314V	site-directed mutagenesis, the mutation results in decreased enzymatic activities towards both the substrates salicylic acid (SA) over benzoic acid (BA), but not in a substrate switch	757984	
2.1.1.274	additional information	an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout	-, 757709	
2.1.1.274	additional information	silencing of gene NbSAMT, quantitative real-time PCR defense-related genes expression analysis	736841	
2.1.1.274	Y147S	reduced methylation of benzoic acid and 3-hydroxybenzoic acid but slightly increased activity towards jasmonic acid	723402	
2.1.1.274	Y147S/M150H	significant increase in the ability to turn over jasmonic acid and vanillic acid	723402	
2.1.1.274	Y147S/M150H/F347Y	slightly increased activity towards short-chain carboxylic acids and jasmonic acid	723402	
2.1.1.274	Y147S/M150H/F347Y/N349I	highly increased activity towards short-chain carboxylic acids and aromatic acids like hydroxybenzoic acids, vanillic acid, jasmonic acid, cinnamic acid, 4-coumaric acid and caffeic acid, broadest substrate spectrum	723402	
2.1.1.274	Y147S/M150H/I225Q/F347Y	greatest specific activities against 3-hydroxybenzoic acid, vanillic acid and jasmonic acid but reduced activity towards salicylic acid	723402