1.5.5.2	D370A	mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601	658211	
1.5.5.2	D370A/Y540F	mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601	658211	
1.5.5.2	F10E/L12E	construction of a variant with a more polar N-terminus, mutant F10E/L12E, because Thermus thermophilus ProDH (TtProDH), produced through fusion with maltose-binding protein (MBP) appears to be prone to aggregation. Preparation of TtProDH mutant variant DELTAABC, which lacks helices alphaA, alphaB and alphaC, the mutant shows no electron density for an AMP moiety of the cofactor	-, 743834	
1.5.5.2	K110A	inactive	724389	
1.5.5.2	L432P	mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability	658033	
1.5.5.2	additional information	expression of PRODH/POX in DLD-1 colorectal cancer cells significantly decreases basal and maximal respiration at both 3 and 5 days, and proline addition exacerbates this effect. PRODH/POX-dependent inhibition of respiration can be modulated by the duration of PRODH/POX expression and the availability of proline	741588	
1.5.5.2	additional information	generation of an enzyme-deficient prodh1 mutant	741858	
1.5.5.2	additional information	mutants deficient in proline dehydrogenase activity	392572	
1.5.5.2	additional information	the truncated form of Pseudomonas putida PutA shows proline dehydrogenase activity	-, 742641	
1.5.5.2	additional information	truncated enzyme containing residues 86-601 and only four Trp residues. Substantial conformational changes of truncated protein upon addition of proline	671977	
1.5.5.2	W194F	mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 50%	671977	
1.5.5.2	W194F/W211F	mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 95%	671977	
1.5.5.2	W211F	mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 80%	671977	
1.5.5.2	Y203F	the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme	724389	
1.5.5.2	Y540A	a construct consisting of residue 86-630 of PutA is used	696361	
1.5.5.2	Y540F	mutant form of PutA86-601. The ratio of turnover-number to Km-value is 5.8fold lower than the ratio of the wild-type PutA86-601	658211	
1.5.5.2	Y540S	a construct consisting of residue 86-630 of PutA is used	696361