1.3.7.4	D116N	mutant still retains the ability of substrate binding, but with only 1.5% relative activity of wild type protein	687760	
1.3.7.4	D123N	site-directed mutagenesis, the mutated enzyme retains biliverdin IXalpha binding activity and radical formation activity, whereas the PPhiB formation activity is negligible	763272	
1.3.7.4	D146N	mutant completely loses catalytic activity and also the ability of biliverdin binding	687760	
1.3.7.4	D256E	mutant retains only partial activity	687760	
1.3.7.4	D263N	site-directed mutagenesis, the mutated enzyme retains biliverdin IXalpha binding activity and radical formation activity, whereas the PPhiB formation activity is negligible	763272	
1.3.7.4	E110Q	site-directed mutagenesis, the mutant shows 321.7% of wild-type activity	712484	
1.3.7.4	E187Q	site-directed mutagenesis, the mutant shows 20.3% of wild-type activity	712484	
1.3.7.4	H259Q	site-directed mutagenesis, the mutant shows 123.4% of wild-type activity	712484	
1.3.7.4	K183Q	site-directed mutagenesis, the mutant shows 24.6% of wild-type activity	712484	
1.3.7.4	K255Q	site-directed mutagenesis, the mutant shows 11.7% of wild-type activity	712484	
1.3.7.4	K263Q	site-directed mutagenesis, the mutant shows 25.8% of wild-type activity	712484	
1.3.7.4	additional information	identification of elongated hypocotyl-1 (elh1) mutant C1238, comparison of mutant seedlings with wild-type CCMC seedlings, phenotype, overview. Analysis of the effect of CsHY2 mutation on the function of phys, the expression dynamics of cucumber PHYs genes (CsPHYA1, CsPHYA2, CsPHYB, CsPHYC, and CsPHYE). The 35S:CsHY2-EGFP plasmid construct is transformed into Agrobacterium tumefaciens strain GV3101. Arabidopsis hy2-1 recessive homozygous mutants are transformed and the phenotype is analyzed. The expression level of CsHY2 in mutant is the highest in male and female flowers, followed in order by stem, true leaves, root, cotyledon, and fruit. There is no significant difference in CsHY2 expression in all these organs except cotyledons, true leaves, and stem between wild-type and mutant elh1	763754	
1.3.7.4	additional information	the flooding (rf) mutation is analysed in the tomato gene AUREA (AU), it not only led to leaf chlorosis but also causes the spontaneous formation of adventitious roots (ARs) on stems. Under flooding stress, au mutants extensively form ARs along the stem, while wild-type plants produce ARs only at the root-shoot junction. After submergence for 7 days, the resistant to flooding (rf) mutants show higher sensitivity to flooding regarding AR initiation compared to wild-type. Mutation of the AU gene appears to have minimal impact on tomato growth and fruit yield. The flooding resistance of rf mutants mainly results from the accumulation of heme and enhanced HO-1 activity, both of which function in the phytochrome synthesis pathway. In addition, the exchange of substances, including heme, between leaves and stems accounts for AR formation on stems	763128	
1.3.7.4	N133	mutant produces only partial activity	687760	
1.3.7.4	R200Q	site-directed mutagenesis, the mutant shows 12.5% of wild-type activity	712484	
1.3.7.4	R200Q/R264Q	site-directed mutagenesis, the mutant shows 11.9% of wild-type activity	712484	
1.3.7.4	R252Q	mutant loses catalytic activity and the ability of substrate binding	687760	
1.3.7.4	R264Q	site-directed mutagenesis, the mutant shows 18.9% of wild-type activity	712484	
1.3.7.4	V121A	site-directed mutagenesis, single-turnover analysis demonstrates that the V121A mutated protein is slightly slower, although it produces 3Z/E-PPhiB on wild-type level, whereas no activity is detected in the V121A mutated protein in the steady-state analysis	763272