1.14.20.15 A118D mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity -, 748759 1.14.20.15 A118D suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity -, 746579 1.14.20.15 A118E mutant enzyme binds Fe(II), but the mutations completely abrogates chlorination activity -, 748759 1.14.20.15 A118Q suppressed H-bonding interaction of Arg254 with Cl-Fe(III)-OH and suppressed chlorination activity -, 746579 1.14.20.15 E102A mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity -, 747733 1.14.20.15 E105A mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity 747733 1.14.20.15 F121A mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity 747733 1.14.20.15 F124A mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity 747733 1.14.20.15 F195A mutation abolishes the production of syringomycin, as shown by a lack of antifungal activity 747733 1.14.20.15 F196A inactive mutant enzyme -, 747733 1.14.20.15 F201A inactive mutant enzyme 747733 1.14.20.15 N123A strong decrease in antifungal activity. The residual activity is approximately 26-30% 747733 1.14.20.15 N126A strong decrease in antifungal activity. The residual activity is approximately 26-30% 747733 1.14.20.15 R247A suppressed chlorination activity -, 746579 1.14.20.15 R247Q suppressed chlorination activity -, 746579 1.14.20.15 R40A mutant enzyme shows 26% residual activity -, 747733 1.14.20.15 R44A mutant enzyme shows 26% residual activity 747733 1.14.20.15 Y107A mutant enzyme shows 75% residual activity -, 747733 1.14.20.15 Y110A mutant enzyme shows 74% residual activity 747733 1.14.20.15 Y178A mutant enzyme shows 23-26% activity -, 747733 1.14.20.15 Y184A mutant enzyme shows 23-26% activity 747733