1.14.11.6 D191A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 D215S mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels 689246 1.14.11.6 D216N site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 D393A site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain 700501 1.14.11.6 D416A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect 700084 1.14.11.6 E122A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 F292A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 745921 1.14.11.6 H189A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 H189A/D191A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 H213S mutant proteins fail to restore the J-level in the JBP1 null trypanosomes to wild-type levels 689246 1.14.11.6 H214A site-directed mutagenesis, the mutant shows highly reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 H239A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 H271A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 745921 1.14.11.6 H391A site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2, expression of the TH mutant H319A fails to stimulate base J formation 700501 1.14.11.6 H414A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect 700084 1.14.11.6 H441A site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain 700501 1.14.11.6 H465A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect 700084 1.14.11.6 L192A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 L223A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 additional information construction of a C-terminally truncated enzyme mutant NcT7H, comprising residues 1-299 745921 1.14.11.6 additional information inactivation of both of the LtJBP2 alleles by conventional knock-out strategy, the major phenotypes of the LtJBP2-/- cells, showing reduced levels of J and serious growth defects, can be completely reversed by the presence of an ectopic copy of LtJBP2 700084 1.14.11.6 N187D site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 697315 1.14.11.6 N250D site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 697315 1.14.11.6 N293D site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 697315 1.14.11.6 N294A site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme 745921 1.14.11.6 N87A site-directed mutagenesis, the mutant shows similar activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 R190A site-directed mutagenesis, the mutant shows highly reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 R190K site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 R255A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 R285C site-directed mutagenesis, inactive active site mutant 697315 1.14.11.6 R286A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 R286K site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 R445A site-directed mutagenesis, mutation inside the catalytic domain, procyclic cells expressing mutant versions of JBP2 inside the catalytic domain 700501 1.14.11.6 R478A site-directed mutagenesis, cells expressing the LtJBP2 mutant exhibits a serious growth defect 700084 1.14.11.6 S288A site-directed mutagenesis, the mutant shows similar activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 V259A mutation abrogates the ability of JBP1 to complement JBP1 null mutants 689246 1.14.11.6 V273A site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 V459A site-directed mutagenesis, control substitution of a residue outside the catalytic domain 700501 1.14.11.6 V482A/L548Q site-directed and random mutagenesis, the mutant can complement the JBP2 knockout mutant strain 700084 1.14.11.6 Y194F site-directed mutagenesis, the mutant shows reduced activity with thymine and altered substrate specificity compared to the wild-type enzyme 745921 1.14.11.6 Y217A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 745921 1.14.11.6 Y217F site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme 745921