1.13.11.55 C101/104A enzyme with decreased specific activity 664994 1.13.11.55 C101/104S enzyme with decreased specific activity 664994 1.13.11.55 C101A about 10% residual activtiy for both oxygenase and reductase activity 765288 1.13.11.55 C101A enzyme with decreased specific activity 664994 1.13.11.55 C101A iron content is 49% of that of the recombinant wild-type enzyme, oxygenase activity is 12.8% of the activity of recombinant wild-type enzyme, reductase activity is 10.3% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C101A/C104A iron content is 124% of that of the recombinant wild-type enzyme, oxygenase activity is 9.7% of the activity of recombinant wild-type enzyme, reductase activity is 15.3% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C101S 1.95 mol iron content per mol subunit, cysteine residue is essential for activity 697350 1.13.11.55 C101S 98.4% loss of activity -, 663712 1.13.11.55 C101S enzyme with decreased specific activity and a proportional decrease in iron content 664994 1.13.11.55 C101S mutant enzyme contains no iron, oxygenase activity is 1.04% of the activity of recombinant wild-type enzyme, reductase activity is 0.5% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C101S mutant with reduced activity -, 663712 1.13.11.55 C101S mutation of any cysteine residues (C31S, C101S, and C104S) at the active site leads to complete loss of SOR catalytic ability 684915 1.13.11.55 C101S site-directed mutagenesis, 10% reamining activity compared to the wild-type enzyme -, 725890 1.13.11.55 C101S/C104S iron content is 89% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 27.9% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C104A about 10% residual activtiy for both oxygenase and reductase activity 765288 1.13.11.55 C104A enzyme with decreased specific activity 664994 1.13.11.55 C104A iron content is 42% of that of the recombinant wild-type enzyme, oxygenase activity is 11.8% of the activity of recombinant wild-type enzyme, reductase activity is 5.5% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C104S 2.4 mol iron content per mol subunit, cysteine residue is essential for activity 697350 1.13.11.55 C104S 99.2% loss of activity -, 663712 1.13.11.55 C104S enzyme with decreased specific activity 664994 1.13.11.55 C104S iron content is 67% of that of the recombinant wild-type enzyme, oxygenase activity is 19.8% of the activity of recombinant wild-type enzyme, reductase activity is 14.3% of the activity of recombinant wild-type enzyme 664994 1.13.11.55 C104S mutant with reduced activity -, 663712 1.13.11.55 C104S mutation of any cysteine residues (C31S, C101S, and C104S) at the active site leads to complete loss of SOR catalytic ability 684915 1.13.11.55 C104S site-directed mutagenesis, 47% reamining activity compared to the wild-type enzyme -, 725890 1.13.11.55 C113A site-directed mutagenesis, almost inactive mutant -, 742795 1.13.11.55 C116A site-directed mutagenesis, almost inactive mutant -, 742795 1.13.11.55 C31A complete loss of activity -, 663712, 765288 1.13.11.55 C31A inactive enzyme 664994 1.13.11.55 C31A inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme 664994 1.13.11.55 C31A mutant with reduced activity 663712 1.13.11.55 C31A site-directed mutagenesis, inactive mutant 742795 1.13.11.55 C31S 1.86 mol iron content per mol subunit, cysteine residue is essential for activity 697350 1.13.11.55 C31S complete loss of activity 663712 1.13.11.55 C31S inactive enzyme 664994 1.13.11.55 C31S inactive mutant enzyme, iron content is similar to that of recombinant wild-type enzyme 664994 1.13.11.55 C31S mutant with reduced activity 663712 1.13.11.55 C31S mutation of any cysteine residues (C31S, C101S, and C104S) at the active site leads to complete loss of SOR catalytic ability 684915 1.13.11.55 C31S site-directed mutagenesis, inactive mutant 725890 1.13.11.55 C44A site-directed mutagenesis, inactive mutant -, 742795 1.13.11.55 E114A complete loss of activity 765288 1.13.11.55 E114A inactive enzyme with no iron incorporated 664994 1.13.11.55 E114A mutation results in inactive enzyme with no measurable iron found 664994 1.13.11.55 E114A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 725890 1.13.11.55 E114D enzyme with 1% of wild type activity 664994 1.13.11.55 E114D iron content is 4.4% of wild-type value, sulfur-oxidizing and sulfur-reducing activity is about 1% of the activity of activity of the recombinant wild-type enzyme 664994 1.13.11.55 E129A site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0.81 mol iron content per mol subunit 697350 1.13.11.55 F133A site-directed mutagenesis of a tetramer channel residue, the mutant shows reduced activity compared to the wild-type enzyme 725074 1.13.11.55 F133A/F141A site-directed mutagenesis of a tetramer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 F141A site-directed mutagenesis of a tetramer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 H166A site-directed mutagenesis of the zinc site residue, the mutant shows reduced activity compared to the wild-type enzyme 725074 1.13.11.55 H277A site-directed mutagenesis of the zinc site residue, the mutant shows activity similar to the wild-type enzyme 725074 1.13.11.55 H86A complete loss of activity 765288 1.13.11.55 H86A inactive enzyme with no iron incorporated 664994 1.13.11.55 H86A mutation results in inactive enzyme with no measurable iron found 664994 1.13.11.55 H86A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme -, 725890 1.13.11.55 H86F site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0 mol iron content per mol subunit 697350 1.13.11.55 H90A complete loss of activity 765288 1.13.11.55 H90A inactive enzyme with no iron incorporated 664994 1.13.11.55 H90A mutation results in inactive enzyme with no measurable iron found 664994 1.13.11.55 H90A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme -, 725890 1.13.11.55 H90F site-directed mutagenesis, no change of the secondary structure, but mutant is completely inactive, 0.6 mol iron content per mol subunit 697350 1.13.11.55 His86F mutation results in a dramatic reduction in SOR activity 684915 1.13.11.55 His90F mutation results in a dramatic reduction in SOR activity 684915 1.13.11.55 M296V site-directed mutagenesis of the active site pore residue, the mutant shows slightly increased activity compared to the wild-type enzyme 725074 1.13.11.55 M297A site-directed mutagenesis of the active site pore residue, the mutant shows reduced activity compared to the wild-type enzyme 725074 1.13.11.55 MM296/297TT site-directed mutagenesis of the active site pore residue, the mutant shows reduced activity compared to the wild-type enzyme 725074 1.13.11.55 MM296/297VT site-directed mutagenesis of the active site pore residue, the mutant shows reduced activity compared to the wild-type enzyme 725074 1.13.11.55 additional information generation of an enzyme mutant lacking the sulfur oxygenase reductase gene sor, comparative transcriptome analysis, microarrays and real-time quantitative PCR, of the wild-type and the DELTAsor mutant, growth analysis on sulfur or K2S4O6 as the sole substrates reveals that the mutant has an obvious growth advantage compared to the wild-type strain and its maximum cell concentration is 70% higher than the wild-type, overview -, 726299 1.13.11.55 additional information modeling of active site pore mutants based on the wild-type structure, overview 725074 1.13.11.55 additional information mutations of putative SOR active site residues, C31, C101, C104, H86 and H90, and E114: replacement of any cysteine residues reduced SORactivity by 53-100%, while the mutants of H86A, H90A and E114A lost their enzyme activities largely, only remaining 20%, 19% and 32% activity of the wild type SOR respectively -, 725890 1.13.11.55 R99A site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 R99I site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 S226A site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 S226I site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 S226L site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074 1.13.11.55 S226T site-directed mutagenesis of a trimer channel residue, the mutant shows increased activity compared to the wild-type enzyme 725074