1.13.11.54 D101A about 60% of wild-type activity -, 685212 1.13.11.54 E100A about 2% of wild-type activity. E100 is not essential for metal binding -, 685212 1.13.11.54 E102A no catalytic activity 685212 1.13.11.54 E91A no activity -, 673849 1.13.11.54 E94A no activity 673849 1.13.11.54 E95A about 4% of wild-type activity 685212 1.13.11.54 H140A no catalytic activity -, 685212 1.13.11.54 H140F no catalytic activity 685212 1.13.11.54 H96A no catalytic activity -, 685212 1.13.11.54 H98A no catalytic activity 685212 1.13.11.54 H98S mutation results in the formation of a stable soluble protein that while structurally different from ARD shows a high degree of similarity to the ARD' enzyme 675408 1.13.11.54 H98S no catalytic activity. Mutant exhibits little affinity for either Ni2+ or Fe2+, indicating that His 98 is likely involved in binding both metals -, 685212 1.13.11.54 additional information usage of three tDNA insertion mutant alleles, ard1-1 (SALK_119327), ard1-2 (GABI_595C04), and ard1-3 (SALK_034308). -, 725438