1.10.3.3 A389N slightly reduced thermostability 439941 1.10.3.3 E177K slightly reduced thermostability 439941 1.10.3.3 E540L slightly reduced thermostability 439941 1.10.3.3 H383Y slightly reduced thermostability 439941 1.10.3.3 L539E slightly increased thermostability 439941 1.10.3.3 M542L strongly reduced thermostability 439941 1.10.3.3 additional information construction of RNAi knockout plants lacking AAO activity, phenotype, overview. RNAi tomato lines have reduced ascorbate oxidase transcript and activity in leaves. Stomatal conductance is increased in transgenic ascorbate oxidase lines -, 726138 1.10.3.3 additional information construction of three transgenic enzyme overexpressing lines R50, R60, and R68, selected on the basis of enzyme expression and single transgene copy number, the overexpression has a slight effect on chlorophyll and carotinoid contents, and on sensitivity to oxidative stress, phenotypes, overview 675145 1.10.3.3 additional information construction of transgenic Nicotiana tabacum plants overexpressing the enzyme in sense orientation, lines P221 and P372, the apoplast ascorbic acid pool is only about 3% reduced in enzyme 'in sense' lines, ascorbic acid oxidase sense leaves exhibit significantly lower dehydroascorbate reductase and ascorbate peroxidase activities than wild-type and antisense leaves, phenotype and effects on enzyme regulation, overview 676611 1.10.3.3 additional information mutant with T-DNA inserted into enzyme gene, delay in flowering time, at high salinity, increase in percentage of germination, photosynthetic activity and seed yield 656412 1.10.3.3 P190I reduced thermostability 439941 1.10.3.3 P190I/V193P pH optimum shifted to pH 4.5, slightly reduced thermostability 439941 1.10.3.3 Q183R similar properties as wild-type 439941 1.10.3.3 Q382G slightly higher thermostability 439941 1.10.3.3 R194Q similar properties as wild-type 439941 1.10.3.3 R387A slightly reduced thermostability 439941 1.10.3.3 T527A similar properties as wild-type 439941 1.10.3.3 V185K slightly reduced thermostability 439941 1.10.3.3 V185K/V188R similar properties as wild-type 439941 1.10.3.3 V188R/P190I no detectable activity 439941 1.10.3.3 V193E pH optimum shifted to pH 4.5, very thermolabile 439941 1.10.3.3 V193F slightly reduced thermostability 439941 1.10.3.3 V193G very thermolabile 439941 1.10.3.3 V193H slightly reduced thermostability 439941 1.10.3.3 V193K slightly reduced thermostability 439941 1.10.3.3 V193P pH optimum shifted to pH 5.0 439941 1.10.3.3 V193T similar properties as wild-type 439941 1.10.3.3 V385K reduced thermostability 439941 1.10.3.3 V543H very thermolabile 439941 1.10.3.3 W189H no detectable activity 439941 1.10.3.3 W386H reduced thermostability 439941