1.1.1.87	A80del	site-directed mutagenesis, the mutant shows altered substrate specificity preferring isocitrate to homoisocitrate, it is unable to oxidize 3-isopropylmalate, the specificity is similar to the enzyme from Thermus thermophilus	660918	
1.1.1.87	D271N	site-directed mutagenesis, mutation of a metal ion ligand and binding determinant for Mg2+, to N. The mutant enzyme shows a decrease of 520fold in V and V/Km_Mg2+, suggesting that the same step(s) limit the reaction at limiting and saturating MgHIc concentrations	739958	
1.1.1.87	I82N	the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate compared to the wild type enzyme and has no activity with 3-isopropylmalate	-, 760522	
1.1.1.87	K206M	site-directed mutagenesis, inactive mutant	739958	
1.1.1.87	K206M	site-directed mutagenesis, the active site mutant shows about 2400fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly	696350	
1.1.1.87	L81P	the mutant shows decreased catalytic efficiency with homoisocitrate, isocitrate and 3-isopropylmalate compared to the wild type enzyme	-, 760522	
1.1.1.87	L83R	the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate compared to the wild type enzyme and has no activity with 3-isopropylmalate	-, 760522	
1.1.1.87	additional information	modulation of the broad substrate specificity of the trifunctional enzyme through site-directed mutagenesis, overview	660918	
1.1.1.87	additional information	trex mutant allele, T to C exchange at nucleotide 251. Midgestation lethal mutant which displays craniofacial, limb, and organ abnormalities due to insufficient retinoic acid signaling	686950	
1.1.1.87	R85V	complete loss of activity with isocitrate, significant activity with 3-isopropylmalate, no effect on activity with homoisocitrate	-, 656127	
1.1.1.87	R87T	site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate	660918	
1.1.1.87	R87V	site-directed mutagenesis, the mutant oxidizes homoisocitrate, but not isocitrate and 3-isopropylmalate	660918	
1.1.1.87	S80A	the mutant shows decreased catalytic efficiency with homoisocitrate, isocitrate and 3-isopropylmalate compared to the wild type enzyme	-, 760522	
1.1.1.87	T71V	the mutant shows decreased catalytic efficiency with homoisocitrate and isocitrate, as well as increased catalytic efficiency with 3-isopropylmalate compared to the wild type enzyme	-, 760522	
1.1.1.87	V135M	site-directed mutagenesis, tetramer-to-dimer structural transition enhances the activity with isocitrate 1.6fold	669109	
1.1.1.87	Y150F	site-directed mutagenesis, inactive mutant	739958	
1.1.1.87	Y150F	site-directed mutagenesis, the active site mutant shows about 680fold reduced activity compared to the wild-type enzyme, the Km for HIc does not change significantly	696350