1.1.1.248 D107A increase in Km, 2fold decrease in kcat 698937 1.1.1.248 D107A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 F104A increase in kcat 698937 1.1.1.248 F104A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 F104A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 F104A/I275A shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type 698937 1.1.1.248 F104A/I275A substitution of Phe104 in the substrate-binding pocket, and Ile275 under the flap domain, the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 I275A increase in Km, shows an increase of velocity by a factor of 2.3 698937 1.1.1.248 I275A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 I275V the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 K186V moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type 698937 1.1.1.248 K186V the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 K240E site-directed mutagenesis, inactive mutant 689571 1.1.1.248 L185A increase in Km, decrease in kcat 698937 1.1.1.248 L185A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L185S the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L185V the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L266A decrease in kcat 698937 1.1.1.248 L266A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 L266A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L266S site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 L266V site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 M271A decrease in kcat 698937 1.1.1.248 M271A increase in Km 698937 1.1.1.248 M271A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 M271T site-directed mutagenesis, inactive mutant 689571 1.1.1.248 additional information specific virus-induced gene silencing as a functional genomics tool to investigate the regulation of morphine biosynthesis via a systematic reduction in enzyme levels responsible for the final six steps in the pathway, overview. Reduced SalR protein levels correlate with lower morphine levels and a substantial increase in the accumulation of salutaridine 720707 1.1.1.248 N152A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 N272A increase in Km, decrease in kcat 698937 1.1.1.248 N272A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 N272T site-directed mutagenesis, inactive mutant 689571 1.1.1.248 R44E site-directed mutagenesis, the mutant enzyme shows altered cofactor specificity and utilizes also NADH in contrast to the wild-type enzyme 689571 1.1.1.248 R48E site-directed mutagenesis, the mutant enzyme shows altered cofactor specificity and utilizes also NADH in contrast to the wild-type enzyme 689571 1.1.1.248 S180A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme 689571 1.1.1.248 S181A moderate effects on substrate affinity, increase in kcat by 50% 698937 1.1.1.248 S181A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 T182A moderate effects on substrate affinity 698937 1.1.1.248 T182A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 V106A moderate effects on substrate affinity, increase in kcat by 90% 698937 1.1.1.248 V106A site-directed mutagenesis, the mutant shows about 2fold increased activity compared to the wild-type enzyme 689571 1.1.1.248 V106A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 Y236F site-directed mutagenesis, inactive mutant 689571