1.1.1.248 K186V moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type 698937 1.1.1.248 K186V the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 K240E site-directed mutagenesis, inactive mutant 689571 1.1.1.248 L185A increase in Km, decrease in kcat 698937 1.1.1.248 L185A the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L185S the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L185V the mutant shows altered kinetics compared to the wild-type enzyme 725460 1.1.1.248 L266A decrease in kcat 698937 1.1.1.248 L266A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 689571 1.1.1.248 L266A the mutant shows altered kinetics compared to the wild-type enzyme 725460