5.5.1.32 D214A mutation of active site residue, complete loss of activity 745848 5.5.1.32 D214E mutation of active site residue, complete loss of activity 745848 5.5.1.32 D214N mutation of active site residue, complete loss of activity 745848 5.5.1.32 F138S substitution to the corresponding serine residue from FimC5, mutant produces a mixture of wild-type major product, hapalindole, and the FimC5 major product, fischerindole 745848 5.5.1.32 W73M/K132M introduction of methionine resiudes for crystallization 745848 5.5.1.32 Y101F/F138S substitution to the corresponding residues from FimC5, mutant produces a mixture of wild-type major product, hapalindole, and the FimC5 major product, fischerindole 745848 5.5.1.32 Y101S mutant shows reduces activity 745848