4.2.1.108 S23A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 T40A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 T41A site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme 749081 4.2.1.108 W21A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 Y52A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 Y85A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 Y85F site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081 4.2.1.108 Y85W site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 749081