3.1.3.56 R350A increased Km 646396 3.1.3.56 T16A 100% of activity 646400 3.1.3.56 V388A traces of activity 646400 3.1.3.56 W234A site-directed mutagenesis, mutation in the bulky and hydrophobic loop, the mutant is only active with 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate 751120 3.1.3.56 W281A site-directed mutagenesis, mutation in the bulky and hydrophobic loop, inactive mutant 751120