3.1.3.56 DELTA1-311 no significant influence on localization of enzyme 646399 3.1.3.56 DELTA1-433 enzyme mainly in cytoplasm 646399 3.1.3.56 DELTA407-411 predominantly soluble 646395 3.1.3.56 DELTA725-1001 enzyme exclusively cytoplasmic 646399 3.1.3.56 E185A site-directed mutagenesis, mutation in the Flex-loop, the mutant shows altered substrate specificities and activities compared to the wild-type enzyme, overview -, 751120 3.1.3.56 E55A no activity 646400 3.1.3.56 E55Q no activity 646400 3.1.3.56 E597Q site-directed mutagenesis, the mutant enzyme shows an over 140fold increased catalytic efficiency and a 2.4fold reduced affinity for Mg2+ compared to the wild-type enzyme 665575 3.1.3.56 E631A no activity 646400 3.1.3.56 F246A site-directed mutagenesis, mutation in the bulky and hydrophobic loop, inactive mutant 751120