6.1.1.1	tyrosine-tRNA ligase	-	
6.1.1.10	methionine-tRNA ligase	-	
6.1.1.11	serine-tRNA ligase	-	
6.1.1.12	aspartate-tRNA ligase	-	
6.1.1.13	D-alanine-poly(phosphoribitol) ligase	-	
6.1.1.14	glycine-tRNA ligase	-	
6.1.1.15	proline-tRNA ligase	-	
6.1.1.16	cysteine-tRNA ligase	-	
6.1.1.17	glutamate-tRNA ligase	-	
6.1.1.18	glutamine-tRNA ligase	-	
6.1.1.19	arginine-tRNA ligase	-	
6.1.1.2	tryptophan-tRNA ligase	-	
6.1.1.20	phenylalanine-tRNA ligase	-	
6.1.1.21	histidine-tRNA ligase	-	
6.1.1.22	asparagine-tRNA ligase	-	
6.1.1.23	aspartate-tRNAAsn ligase	-	
6.1.1.24	glutamate-tRNAGln ligase	-	
6.1.1.25	lysine-tRNAPyl ligase	deleted	
6.1.1.26	pyrrolysine-tRNAPyl ligase	-	
6.1.1.27	O-phospho-L-serine-tRNA ligase	-	
6.1.1.3	threonine-tRNA ligase	-	
6.1.1.4	leucine-tRNA ligase	-	
6.1.1.5	isoleucine-tRNA ligase	-	
6.1.1.6	lysine-tRNA ligase	-	
6.1.1.7	alanine-tRNA ligase	-	
6.1.1.8	D-alanine-sRNA sythetase	deleted	
6.1.1.9	valine-tRNA ligase	-	
6.1.1.B3	glutamyl-Q-tRNA(Asp) ligase	preliminary BRENDA-supplied EC number	
6.1.2.1	D-alanine-(R)-lactate ligase	-	
6.1.2.2	nebramycin 5' synthase	-	
6.1.3.1	olefin beta-lactone synthetase	-	
6.2.1.1	acetate-CoA ligase	-	
6.2.1.10	carboxylic acid-CoA ligase (GDP-forming)	-	
6.2.1.11	biotin-CoA ligase	-	
6.2.1.12	4-coumarate-CoA ligase	-	
6.2.1.13	acetate-CoA ligase (ADP-forming)	-	
6.2.1.14	6-carboxyhexanoate-CoA ligase	-	
6.2.1.15	arachidonate-CoA ligase	-	
6.2.1.16	acetoacetate-CoA ligase	-	
6.2.1.17	propionate-CoA ligase	-	
6.2.1.18	citrate-CoA ligase	-	
6.2.1.19	long-chain-fatty-acid-protein ligase	-	
6.2.1.2	medium-chain acyl-CoA ligase	-	
6.2.1.20	long-chain-fatty-acid-[acyl-carrier-protein] ligase	-	
6.2.1.21	phenylacetate-CoA ligase	deleted, included in EC 6.2.1.30	
6.2.1.22	[citrate (pro-3S)-lyase] ligase	-	
6.2.1.23	dicarboxylate-CoA ligase	-	
6.2.1.24	phytanate-CoA ligase	-	
6.2.1.25	benzoate-CoA ligase	-	
6.2.1.26	O-succinylbenzoate-CoA ligase	-	
6.2.1.27	4-hydroxybenzoate-CoA ligase	-	
6.2.1.28	3alpha,7alpha-Dihydroxy-5beta-cholestanate-CoA ligase	-	
6.2.1.29	3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate-CoA ligase	deleted, identical to EC 6.2.1.7	
6.2.1.3	long-chain-fatty-acid-CoA ligase	-	
6.2.1.30	phenylacetate-CoA ligase	-	
6.2.1.31	2-furoate-CoA ligase	-	
6.2.1.32	anthranilate-CoA ligase	-	
6.2.1.33	4-chlorobenzoate-CoA ligase	-	
6.2.1.34	trans-feruloyl-CoA synthase	-	
6.2.1.35	acetate-[acyl-carrier protein] ligase	-	
6.2.1.36	3-hydroxypropionyl-CoA synthase	-	
6.2.1.37	3-hydroxybenzoate-CoA ligase	-	
6.2.1.38	(2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA synthase	-	
6.2.1.39	[butirosin acyl-carrier protein]-L-glutamate ligase	-	
6.2.1.4	succinate-CoA ligase (GDP-forming)	-	
6.2.1.40	4-hydroxybutyrate-CoA ligase (AMP-forming)	-	
6.2.1.41	3-[(3aS,4S,7aS)-7a-methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate-CoA ligase	-	
6.2.1.42	3-oxocholest-4-en-26-oate-CoA ligase	-	
6.2.1.43	2-hydroxy-7-methoxy-5-methyl-1-naphthoate-CoA ligase	-	
6.2.1.44	3-(methylthio)propionyl-CoA ligase	-	
6.2.1.45	E1 ubiquitin-activating enzyme	-	
6.2.1.46	L-allo-isoleucine-holo-[CmaA peptidyl-carrier protein] ligase	-	
6.2.1.47	medium-chain-fatty-acid-[acyl-carrier-protein] ligase	-	
6.2.1.48	carnitine-CoA ligase	-	
6.2.1.49	long-chain fatty acid adenylyltransferase FadD28	-	
6.2.1.5	succinate-CoA ligase (ADP-forming)	-	
6.2.1.50	4-hydroxybenzoate adenylyltransferase FadD22	-	
6.2.1.51	4-hydroxyphenylalkanoate adenylyltransferase FadD29	-	
6.2.1.52	L-firefly luciferin-CoA ligase	-	
6.2.1.53	L-proline-[L-prolyl-carrier protein] ligase	-	
6.2.1.54	D-alanine-[D-alanyl-carrier protein] ligase	-	
6.2.1.55	E1 SAMP-activating enzyme	-	
6.2.1.56	4-hydroxybutyrate-CoA ligase (ADP-forming)	-	
6.2.1.57	long-chain fatty acid adenylase/transferase FadD23	-	
6.2.1.58	isophthalate-CoA ligase	-	
6.2.1.59	long-chain fatty acid adenylase/transferase FadD26	-	
6.2.1.6	glutarate-CoA ligase	-	
6.2.1.60	marinolic acid-CoA ligase	-	
6.2.1.61	salicylate-[aryl-carrier protein] ligase	-	
6.2.1.62	3,4-dihydroxybenzoate-[aryl-carrier protein] ligase	-	
6.2.1.63	L-arginine-[L-arginyl-carrier protein] ligase	-	
6.2.1.64	E1 NEDD8-activating enzyme	-	
6.2.1.65	salicylate-CoA ligase	-	
6.2.1.66	glyine---[glycyl-carrier protein] ligase	-	
6.2.1.67	L-alanine---[L-alanyl-carrier protein] ligase	-	
6.2.1.68	L-glutamate---[L-glutamyl-carrier protein] ligase	-	
6.2.1.69	L-cysteine-[L-cysteinyl-carrier protein] ligase	-	
6.2.1.7	cholate-CoA ligase	-	
6.2.1.70	L-threonine-[L-threonyl-carrier protein] ligase	-	
6.2.1.71	2,3-dihydroxybenzoate-[aryl-carrier protein] ligase	-	
6.2.1.72	L-serine-[L-seryl-carrier protein] ligase	-	
6.2.1.73	L-tryptophan-[L-tryptophyl-carrier protein] ligase	-	
6.2.1.74	3-amino-5-hydroxybenzoate-[acyl-carrier protein] ligase	-	
6.2.1.75	indoleacetate-CoA ligase	-	
6.2.1.76	malonate-CoA ligase	-	
6.2.1.8	oxalate-CoA ligase	-	
6.2.1.9	malate-CoA ligase	-	
6.2.1.B1	O-succinylbenzoate-CoA ligase (ADP-forming)	preliminary BRENDA-supplied EC number	
6.2.1.B10	2-(imidazol-4-yl)acetate-CoA ligase	preliminary BRENDA-supplied EC number	
6.2.1.B11	carboxylic acid-CoA ligase (NDP-forming)	preliminary BRENDA-supplied EC number	
6.2.1.B13	3-phenylpropionic acid-CoA ligase	preliminary BRENDA-supplied EC number	
6.2.1.B3	OPC-8:CoA ligase	preliminary BRENDA-supplied EC number	
6.2.1.B4	glycine:[acyl-carrier protein] ligase (AMP forming)	preliminary BRENDA-supplied EC number	
6.2.1.B5	L-alanine:[acyl-carrier protein] ligase (AMP forming)	preliminary BRENDA-supplied EC number	
6.2.2.1	thioglycine synthase	-	
6.2.2.2	oxazoline synthase	-	
6.2.2.3	thiazoline synthase	-	
6.3.1.1	aspartate-ammonia ligase	-	
6.3.1.10	adenosylcobinamide-phosphate synthase	-	
6.3.1.11	glutamate-putrescine ligase	-	
6.3.1.12	D-aspartate ligase	-	
6.3.1.13	L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase	-	
6.3.1.14	diphthine-ammonia ligase	-	
6.3.1.15	8-demethylnovobiocic acid synthase	-	
6.3.1.16	carbapenam-3-carboxylate synthetase	transferred to EC 6.3.3.6	
6.3.1.17	beta-citrylglutamate synthase	-	
6.3.1.18	gamma-glutamylanilide synthase	-	
6.3.1.19	prokaryotic ubiquitin-like protein ligase	-	
6.3.1.2	glutamine synthetase	-	
6.3.1.20	lipoate-protein ligase	-	
6.3.1.21	phosphoribosylglycinamide formyltransferase 2	-	
6.3.1.3	phosphoribosyl-glycinamide synthetase	transferred to EC 6.3.4.13	
6.3.1.4	aspartate-ammonia ligase (ADP-forming)	-	
6.3.1.5	NAD+ synthase	-	
6.3.1.6	glutamate-ethylamine ligase	-	
6.3.1.7	4-methyleneglutamate-ammonia ligase	-	
6.3.1.8	glutathionylspermidine synthase	-	
6.3.1.9	trypanothione synthase	-	
6.3.2.1	pantoate-beta-alanine ligase (AMP-forming)	-	
6.3.2.10	UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase	-	
6.3.2.11	carnosine synthase	-	
6.3.2.12	dihydrofolate synthase	-	
6.3.2.13	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase	-	
6.3.2.14	enterobactin synthase	-	
6.3.2.15	UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamino-pimelate-D-alanyl-D-alanine ligase	deleted	
6.3.2.16	D-alanine-alanyl-poly(glycerolphosphate) ligase	-	
6.3.2.17	tetrahydrofolate synthase	-	
6.3.2.18	gamma-glutamylhistamine synthase	-	
6.3.2.19	ubiquitin-protein ligase	deleted. The ubiquitinylation process is now known to be performed by several enzymes in sequence, starting with EC 6.2.1.45 (ubiquitin-activating enzyme E1) and followed by several transfer reactions, including those of EC 2.3.2.23 (E2 ubiquitin-conjugating enzyme) and EC 2.3.2.27 (RING-type E3 ubiquitin transferase)	
6.3.2.2	glutamate-cysteine ligase	-	
6.3.2.20	indoleacetate-lysine synthetase	-	
6.3.2.21	ubiquitin-calmodulin ligase	deleted. The reaction is performed by the sequential action of EC 6.2.1.45 (ubiquitin-activating enzyme E1), several ubiquitin transferases and finally by EC 2.3.2.27 [ubiquitin transferase RING E3 (calmodulin-selective)]	
6.3.2.22	diphthine-ammonia ligase	transferred to EC 6.3.1.14	
6.3.2.23	homoglutathione synthase	-	
6.3.2.24	tyrosine-arginine ligase	-	
6.3.2.25	tubulin-tyrosine ligase	-	
6.3.2.26	N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase	-	
6.3.2.27	aerobactin synthase	deleted, now covered by EC 6.3.2.38 N2-citryl-N6-acetyl-N6-hydroxylysine synthase, and EC 6.3.2.39, aerobactin synthase	
6.3.2.28	L-amino-acid alpha-ligase	transferred to EC 6.3.2.49	
6.3.2.29	cyanophycin synthase (L-aspartate-adding)	-	
6.3.2.3	glutathione synthase	-	
6.3.2.30	cyanophycin synthase (L-arginine-adding)	-	
6.3.2.31	coenzyme F420-0:L-glutamate ligase	-	
6.3.2.32	coenzyme gamma-F420-2:alpha-L-glutamate ligase	-	
6.3.2.33	tetrahydrosarcinapterin synthase	-	
6.3.2.34	coenzyme F420-1:gamma-L-glutamate ligase	-	
6.3.2.35	D-alanine-D-serine ligase	-	
6.3.2.36	4-phosphopantoate-beta-alanine ligase	-	
6.3.2.37	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-D-lysine ligase	-	
6.3.2.38	N2-citryl-N6-acetyl-N6-hydroxylysine synthase	-	
6.3.2.39	aerobactin synthase	-	
6.3.2.4	D-Alanine-D-alanine ligase	-	
6.3.2.40	cyclopeptine synthase	-	
6.3.2.41	N-acetylaspartylglutamate synthase	-	
6.3.2.42	N-acetylaspartylglutamylglutamate synthase	-	
6.3.2.43	[amino group carrier protein]-L-2-aminoadipate ligase	-	
6.3.2.44	pantoate-beta-alanine ligase (ADP-forming)	-	
6.3.2.45	UDP-N-acetylmuramate-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate ligase	-	
6.3.2.46	fumarate-(S)-2,3-diaminopropanoate ligase	-	
6.3.2.47	dapdiamide synthase	-	
6.3.2.48	L-arginine-specific L-amino acid ligase	-	
6.3.2.49	L-alanine-L-anticapsin ligase	-	
6.3.2.5	phosphopantothenate-cysteine ligase (CTP)	-	
6.3.2.50	tenuazonic acid synthetase	-	
6.3.2.51	phosphopantothenate-cysteine ligase (ATP)	-	
6.3.2.52	jasmonoyl-L-amino acid ligase	-	
6.3.2.53	UDP-N-acetylmuramoyl-L-alanine-L-glutamate ligase	-	
6.3.2.54	L-2,3-diaminopropanoate-citrate ligase	-	
6.3.2.55	2-[(L-alanin-3-ylcarbamoyl)methyl]-3-(2-aminoethylcarbamoyl)-2-hydroxypropanoate synthase	-	
6.3.2.56	staphyloferrin B synthase	-	
6.3.2.57	staphyloferrin A synthase	-	
6.3.2.58	D-ornithine-citrate ligase	-	
6.3.2.59	3-methyl-D-ornithine---L-lysine ligase	-	
6.3.2.6	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-	
6.3.2.60	glutamate---[amino group carrier protein] ligase	-	
6.3.2.61	tubulin-glutamate ligase	-	
6.3.2.62	beta-tubulin-glutamate ligase	-	
6.3.2.7	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-L-lysine ligase	-	
6.3.2.8	UDP-N-acetylmuramate-L-alanine ligase	-	
6.3.2.9	UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase	-	
6.3.2.B1	oligomer L-amino acid ligase	deleted, replaced by EC 6.3.2.30	
6.3.2.B10	L-asparagine:L-amino acid ligase	preliminary BRENDA-supplied EC number	
6.3.2.B11	beta-citrylglutamate synthase	preliminary BRENDA-supplied EC number	
6.3.2.B15	3-[[[(2R,3R)-3-carboxyoxiran-2-yl]carbonyl]amino]-L-alanine-L-valine ligase	preliminary BRENDA-supplied EC number	
6.3.2.B16	L-valine-specific L-amino acid ligase	preliminary BRENDA-supplied EC number	
6.3.2.B19	[LysW]-C-terminal-L-glutamate ligase	preliminary BRENDA-supplied EC number	
6.3.2.B2	murein peptide ligase	preliminary BRENDA-supplied EC number	
6.3.2.B23	3-formyl-tyrosine ligase	preliminary BRENDA-supplied EC number	
6.3.2.B24	beta-tubulin-glutamate ligase	deleted, replaced by EC 6.3.2.62, beta-tubulin-glutamate ligase	
6.3.2.B3	tubulin-glutamate ligase	deleted, replaced by EC 6.3.2.61, tubulin-glutamate ligase	
6.3.2.B4	polyglycylase	preliminary BRENDA-supplied EC number	
6.3.3.1	phosphoribosylformylglycinamidine cyclo-ligase	-	
6.3.3.2	5-formyltetrahydrofolate cyclo-ligase	-	
6.3.3.3	dethiobiotin synthase	-	
6.3.3.4	(carboxyethyl)arginine beta-lactam-synthase	-	
6.3.3.5	O-ureido-D-serine cyclo-ligase	-	
6.3.3.6	carbapenam-3-carboxylate synthase	-	
6.3.3.7	Ni-sirohydrochlorin a,c-diamide reductive cyclase	-	
6.3.4.1	GMP synthase	transferred to EC 6.3.5.2	
6.3.4.10	biotin-[propionyl-CoA-carboxylase (ATP-hydrolysing)] ligase	-	
6.3.4.11	biotin-[methylcrotonoyl-CoA-carboxylase] ligase	-	
6.3.4.12	glutamate-methylamine ligase	-	
6.3.4.13	phosphoribosylamine-glycine ligase	-	
6.3.4.14	biotin carboxylase	-	
6.3.4.15	biotin-[biotin carboxyl-carrier protein] ligase	-	
6.3.4.16	carbamoyl-phosphate synthase (ammonia)	-	
6.3.4.17	formate-dihydrofolate ligase	-	
6.3.4.18	5-(carboxyamino)imidazole ribonucleotide synthase	-	
6.3.4.19	tRNAIle-lysidine synthase	-	
6.3.4.2	CTP synthase (glutamine hydrolysing)	-	
6.3.4.20	7-cyano-7-deazaguanine synthase	-	
6.3.4.21	nicotinate phosphoribosyltransferase	-	
6.3.4.22	tRNAIle2-agmatinylcytidine synthase	-	
6.3.4.23	formate-phosphoribosylaminoimidazolecarboxamide ligase	-	
6.3.4.24	tyramine-L-glutamate ligase	-	
6.3.4.25	2-amino-2'-deoxyadenylo-succinate synthase	-	
6.3.4.3	formate-tetrahydrofolate ligase	-	
6.3.4.4	adenylosuccinate synthase	-	
6.3.4.5	argininosuccinate synthase	-	
6.3.4.6	urea carboxylase	-	
6.3.4.7	ribose-5-phosphate-ammonia ligase	-	
6.3.4.8	imidazoleacetate-phosphoribosyldiphosphate ligase	-	
6.3.4.9	biotin-[methylmalonyl-CoA-carboxytransferase] ligase	-	
6.3.5.1	NAD+ synthase (glutamine-hydrolysing)	-	
6.3.5.10	adenosylcobyric acid synthase (glutamine-hydrolysing)	-	
6.3.5.11	cobyrinate a,c-diamide synthase	-	
6.3.5.12	Ni-sirohydrochlorin a,c-diamide synthase	-	
6.3.5.13	lipid II isoglutaminyl synthase (glutamine-hydrolysing)	-	
6.3.5.2	GMP synthase (glutamine-hydrolysing)	-	
6.3.5.3	phosphoribosylformylglycinamidine synthase	-	
6.3.5.4	asparagine synthase (glutamine-hydrolysing)	-	
6.3.5.5	carbamoyl-phosphate synthase (glutamine-hydrolysing)	-	
6.3.5.6	asparaginyl-tRNA synthase (glutamine-hydrolysing)	-	
6.3.5.7	glutaminyl-tRNA synthase (glutamine-hydrolysing)	-	
6.3.5.8	aminodeoxychorismate synthase	transferred to EC 2.6.1.85	
6.3.5.9	hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing)	-	
6.4.1.1	pyruvate carboxylase	-	
6.4.1.2	acetyl-CoA carboxylase	-	
6.4.1.3	propionyl-CoA carboxylase	-	
6.4.1.4	methylcrotonoyl-CoA carboxylase	-	
6.4.1.5	geranoyl-CoA carboxylase	-	
6.4.1.6	acetone carboxylase	-	
6.4.1.7	2-oxoglutarate carboxylase	-	
6.4.1.8	acetophenone carboxylase	-	
6.4.1.9	coenzyme F430 synthetase	-	
6.5.1.1	DNA ligase (ATP)	-	
6.5.1.2	DNA ligase (NAD+)	-	
6.5.1.3	RNA ligase (ATP)	-	
6.5.1.4	RNA 3'-terminal-phosphate cyclase (ATP)	-	
6.5.1.5	RNA 3'-terminal-phosphate cyclase (GTP)	-	
6.5.1.6	DNA ligase (ATP or NAD+)	-	
6.5.1.7	DNA ligase (ATP, ADP or GTP)	-	
6.5.1.8	3'-phosphate/5'-hydroxy nucleic acid ligase	-	
6.5.1.9	cyclic 2,3-diphosphoglycerate synthase	-	
6.5.1.B1	2'-5' RNA ligase (NTP-independent)	preliminary BRENDA-supplied EC number	
6.5.1.B3	2'-5' RNA ligase (GTP-activated)	preliminary BRENDA-supplied EC number	
6.6.1.1	magnesium chelatase	-	
6.6.1.2	cobaltochelatase	-	
6.7.1.1	3-amino-2-hydroxy-4-methoxybenzoate diazotase	-