4.1.3.30 crystals of the inactive mutant C123S MICL in complex with isocitrate are obtained at room temperature by vapor diffusion in hanging drops. A crystal of C123S MICL in complex with pyruvate and succinate was obtained by overnight incubation of a C123S MICL/isocitrate complex crystal in a solution containing 35% PEG3350, 100 mM pyruvate and 100 mM succinate. This crystal also belongs to space group C2 4.1.3.30 native enzyme and bound with pyruvate/Mg2+, sitting and hanging drop vapor diffusion method 4.1.3.30 sitting drop vapor diffusion method