3.5.99.2	dialyzing the purified enzyme solution against 52% saturated ammonium sulfate solution, two weeks	
3.5.99.2	hanging drop vapor diffusion method	
3.5.99.2	hanging drop vapour diffusion method	
3.5.99.2	purified recombinant C-terminally His6-tagged THI20, hanging drop vapour diffusion method, 10 mg/ml protein in 10 mM Tris buffer, pH 7.6, with 30 mM NaCl, is mixed with 10-14% PEG 8000, 0.2 M calcium acetate, and 0.1 M imidazole, pH 7.5, microseeding, 2-3 days, X-ray diffraction structure determination and analysis at 2.68 A resolution, molecular replacement, modelling	
3.5.99.2	purified recombinant C-terminally Strep-tagged TenA, hanging drop vapour diffusion method, 0.002 ml of 10 mg/ml protein in 100 mM Tris-HCl buffer pH 8.0, is mixed with 0.002 l of reservoir solution containing 0.2 M sodium acetate, 0.1 M Tris-HCl pH 8.5 and 28% w/v PEG 3350, 20°C, 7 days, X-ray diffraction structure determination and analysis at 2.60 A resolution, molecular replacement, modelling	
3.5.99.2	vapor diffusion method, using 0.1 m Tris (pH 8.5), 1.1 M lithium sulfate