3.4.22.70	-	
3.4.22.70	crystals are grown by the hanging-drop technique with a protein concentration of 50 mg/ml in 25 mM MES buffer, pH 6.35. The crystallization conditions include 3.2 M ammonium sulfate, 0.1 M NaCl, and trace amounts of ethylene glycol. Crystal structure of native SrtA, of an active-site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide	
3.4.22.70	hanging drop method of vapor diffusion at 20°C, SrtA residues Val82-Thr249 (catalytic domain)	
3.4.22.70	wild-type enzyme SrtA apo and mutant enzyme C184A bound to LPETG peptide sequence, crystal structure analysis