3.4.21.B6 hanging drop vapor diffusion method. Active prostasin is crystallized, and the structure is determined to 1.45 A resolution. These apoprotein crystals are soaked with nafamostat, allowing the structure of the inhibited acyl-enzyme intermediate structure to be determined to 2.0 A resolution 693192 3.4.21.B6 structure of the extracellular portion of the membrane associated serine protease solved to high resolution in complex with a nonselective d-FFR chloromethyl ketone inhibitor, in an apo form, in a form where the apo crystal is soaked with the covalent inhibitor camostat and in complex with the protein inhibitor aprotinin. It is also crystallized in the presence of the divalent cation Ca2+ 695021 3.4.21.B6 X-ray crystal structures of prostasin with small molecule inhibitors 691307