2.7.7.43	apoenzyme, hanging drop vapor diffusion method, using 200 mM calcium acetate, 0.1 M imidazole pH 8.0, 10% (w/v) PEG 8000	
2.7.7.43	ligand-free and sialic acid-bound enzyme, sitting drop vapor diffusion method, using 0.16 M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 14.4% (w/v) PEG 8000, and 20% (v/v) glycerol. Enzyme in complex with Ca2+ and CTP, sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 6% (w/v) PEG 8000, and 0.2 M calcium acetate	
2.7.7.43	modeling of the closed conformation. Enzyme forms a dimer complex. Amino acids involved in the Neu5Ac binding pocket are Glu162 and Arg165 from monomer B and Gln104, Ser82, Val86, Tyr179, and Phe192 from monomer A	
2.7.7.43	the structure of the C-terminal domain of CMP-sialic acid synthetase is solved to 1.9 A resolution