2.1.1.218	crystal structure PDB ID 5A7T	
2.1.1.218	purified recombinant truncated construct of SaTrm10, lacking the C-terminal domain, native and enriched in selenomethionine (SaTrm10 1-249 and SaTrm10 1-249 SAD, respectively), hanging drop vapour diffusion, for SaTrm10 1-249: mixing of 15 mg/ml protein solution with an equal volume of crystallisation buffer containing 8% ethylene glycol, 0.1 M HEPES, pH 7.5, and 10% PEG 8000, for SaTrm10 1-249 SAD: mixing of 15 mg/ml protein solution with an equal volume of crystallisation buffer containing 2% dioxane, 0.05 M bicine, pH 9.0, and 15% PEG 20000, for full-length enzyme: mixing of 8 mg/ml protein in the presence of 1 mM SAH with an equal volume of crystallisation buffer containing 0.4 M (NH4)2SO4, 0.1 M sodium acetate, pH 4.5, and 15-20% PEG2000MME, several months, 20°C, X-ray diffraction structure determination and analysis at 2.1-2.4 A resolution, molecular replacement and modelling